Root-Derived Oxylipins Promote Green Peach Aphid Performance on Arabidopsis Foliage
Root-Derived Oxylipins Promote Green Peach Aphid Performance on Arabidopsis Foliage
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DOI：
10.1105/tpc.111.094110
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发表时间：
2012-04-01
期刊：
影响因子：
11.6
通讯作者：
Shah, Jyoti
中科院分区：
文献类型：
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作者：
Nalam, Vamsi J.;Keeretaweep, Jantana;Shah, Jyoti
氧脂蛋白作为信号分子参与植物的生长和发育，并参与植物对逆境的防御。在这里，我们表明，氧化脂也促进侵染拟南芥芽韧皮部汁液消耗绿色桃蚜（GPA;桃蚜），一种重要的农业害虫。GPA有困难的饲料从筛元件和挖掘到木质部的脂氧合酶5（lox 5）突变体的LOX活性缺陷的植物。与野生型植物相比，lox 5突变体中GPA性能的这些缺陷伴随着突变体中GPA水含量的降低和GPA群体大小的减小。LOX 5的表达迅速诱导根中的芽感染的GPA响应。与此同时，受GPA定殖的植物的根和叶柄分泌物中LOX 5衍生的氧脂素水平增加。将9-羟基十八碳二烯酸（由LOX 5酶产生的氧化脂素）施用于根恢复了lox 5植物中的水含量和GPA群体大小，从而证实了LOX 5衍生的氧化脂素促进了GPA对叶子的侵染。微嫁接实验表明，GPA的表现在叶子上的影响，在根中的LOX 5基因型，从而证明了根衍生的氧脂在地上器官的定殖昆虫的重要性。
Oxylipins function as signaling molecules in plant growth and development and contribute to defense against stress. Here, we show that oxylipins also facilitate infestation of Arabidopsis thaliana shoots by the phloem sap-consuming green peach aphid (GPA; Myzus persicae), an agronomically important insect pest. GPAs had difficulty feeding from sieve elements and tapping into the xylem of lipoxygenase5 (lox5) mutant plants defective in LOX activity. These defects in GPA performance in the lox5 mutant were accompanied by reduced water content of GPAs and a smaller population size of GPAs in the mutant compared with the wild-type plant. LOX5 expression was rapidly induced in roots in response to infestation of shoots by GPAs. In parallel, levels of LOX5-derived oxylipins increased in roots and in petiole exudates of GPA-colonized plants. Application of 9-hydroxyoctadecadienoic acid (an oxylipin produced by the LOX5 enzyme) to roots restored water content and GPA population size in lox5 plants, thus confirming that a LOX5-derived oxylipin promotes infestation of the foliage by GPAs. Micrografting experiments demonstrated that GPA performance on foliage is influenced by the LOX5 genotype in roots, thus demonstrating the importance of root-derived oxylipins in colonization of aboveground organs by an insect.