Genome Walking
Genome Walking
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DOI：
10.1007/978-1-62703-715-0_12
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Waters, Daniel L. E.
中科院分区：
文献类型：
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作者：
Shapter, Frances M.;Waters, Daniel L. E.
基因组步移是一种用于确定已知DNA序列区域侧翼的未知基因组区域的DNA序列的方法。基因组步移具有在单轮中捕获6-7 kb序列的潜力。非常适合于识别基因启动子区域，其中只有编码区。当靶基因中存在对所表征的物种具有强序列保守性的区域时，基因组步移对于捕获新物种中的同源基因也具有显著的效用。下一代测序技术的使用越来越多，基因组步移的原理将适应计算机模拟方法。然而，对于较小的项目，基于PCR的基因组步移将仍然是表征未知侧翼序列的有效方法。
Genome walking is a method for determining the DNA sequence of unknown genomic regions flanking a region of known DNA sequence. The Genome walking has the potential to capture 6-7 kb of sequence in a single round. Ideal for identifying gene promoter regions where only the coding region. Genome walking also has significant utility for capturing homologous genes in new species when there are areas in the target gene with strong sequence conservation to the characterized species. The increasing use of next-generation sequencing technologies will see the principles of genome walking adapted to in silico methods. However, for smaller projects, PCR-based genome walking will remain an efficient method of characterizing unknown flanking sequence.