Preservation of the delayed-type hypersensitivity response to alloantigen by xyloglucans or oligogalacturonide does not correlate with the capacity to reject ultraviolet-induced skin tumors in mice.
Preservation of the delayed-type hypersensitivity response to alloantigen by xyloglucans or oligogalacturonide does not correlate with the capacity to reject ultraviolet-induced skin tumors in mice.
复制标题
木葡聚糖或低聚半乳糖醛酸对同种抗原的迟发型超敏反应的保留与小鼠排斥紫外线诱导的皮肤肿瘤的能力无关。
DOI：
10.1046/j.1523-1747.2001.00160.x
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Albersheim,P
中科院分区：
文献类型：
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作者：
Strickland,FM;Sun,Y;Darvill,A;Eberhard,S;Pauly,M;Albersheim,P
长期暴露于紫外线辐射抑制T细胞介导的免疫反应，并诱导抑制性T淋巴细胞的形成，防止排斥高抗原性紫外线诱导的小鼠皮肤癌。罗望子木葡聚糖和果胶寡聚半乳糖醛酸可防止小鼠对白色念珠菌和同种异体抗原的迟发型超敏免疫反应的抑制，该免疫反应由单次紫外线照射引起。因此，我们研究了这些聚/低聚糖在慢性紫外线照射期间防止抑制T细胞介导的免疫应答和抑制细胞诱导的能力，以及保持紫外线照射小鼠排斥移植的、高抗原性的紫外线诱导的肿瘤的能力。C3 H/HeN小鼠用15 kJ/m2紫外线B照射，每周3次，持续12周，然后应用多糖/寡糖。对C.分别于治疗后1、6、12 wk检测白念珠菌和同种异体抗原。治疗第12周后，向剩余小鼠注射高抗原性紫外线诱导的同源肿瘤细胞系UV 5497 -5。多糖/寡糖保护对C.白色念珠菌，但没有接触过敏反应，二硝基氟苯长达6周的紫外线照射后，保护下降，抑制细胞观察。与此相反，延迟型过敏反应同种异体抗原保留了整个12周的紫外线照射。尽管对同种异体抗原的免疫保护，移植的肿瘤细胞在所有紫外线照射的动物中同样生长良好。这些结果表明，迟发型超敏反应是异质性和同种异体抗原的迟发型超敏反应是不是一个替代标记紫外线诱导的皮肤肿瘤的排斥反应。
Chronic exposure to ultraviolet radiation suppresses T cell-mediated immune responses and induces the formation of suppressor T lymphocytes that prevent the rejection of highly antigenic ultraviolet-induced skin cancers in mice. Tamarind seed xyloglucans and pectinic oligogalacturonides prevent suppression of delayed-type hypersensitivity immune responses in mice toCandida albicansand alloantigen caused by a single exposure of ultraviolet radiation. We therefore investigated the ability of these poly/oligosaccharides to prevent suppression of T cell-mediated immune responses and suppressor cell induction during chronic ultraviolet irradiation and to preserve the capacity of ultraviolet-irradiated mice to reject a transplanted, highly antigenic, ultraviolet-induced tumor. C3H/HeN mice were treated 3× per week for 12 wk with 15 kJ per m2ultraviolet B radiation followed by application of the polysaccharides/ oligosaccharides. The delayed-type hypersensitivity responses toC. albicansand alloantigen were measured after 1, 6, and 12 wk of treatment. Following the 12th wk of treatment the remaining mice were injected with the highly antigenic ultraviolet-induced, syngeneic tumor cell line UV5497-5. The polysaccharides/oligosaccharides protected delayed-type hypersensitivity responses toC. albicansbut not contact hypersensitivity responses to dinitrofluorobenzene for up to 6 wk of ultraviolet radiation after which protection declined and suppressor cells were observed. In contrast, the delayed-type hypersensitivity response to alloantigen was preserved for the entire 12 wk of ultraviolet irradiation. Despite protection of immunity to alloantigen, the transplanted tumor cells grew equally well in all ultraviolet-irradiated animals. These results indicate that delayed-type hypersensitivity responses are heterogeneous and that delayed-type hypersensitivity to alloantigen is not a surrogate marker for rejection of ultraviolet-induced skin tumors.