The effects of arbuscular mycorrhizal fungi on sex-specific responses to Pb pollution in Populus cathayana
The effects of arbuscular mycorrhizal fungi on sex-specific responses to Pb pollution in Populus cathayana
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丛枝菌根真菌对青杨铅污染性别特异性反应的影响
DOI：
10.1016/j.ecoenv.2014.12.033
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发表时间：
2015-03-01
影响因子：
6.8
通讯作者：
Gao, Shun
中科院分区：
文献类型：
--
作者：
Chen, Lianghua;Hu, Xiangwei;Gao, Shun
利用速生树种修复土壤重金属污染已受到越来越多的关注，尤其是对重金属污染最严重的重金属铅的修复。然而，人们对植物对扩散水平的铅污染的反应知之甚少，需要探索一种更结合的植物释放技术。本研究以分布于某铅锌矿尾矿的胡杨丛枝菌根真菌(Funneliformis Mosseae)为研究对象，研究了在铅污染扩散水平(100 mg Pb2+kg(-1)干土)下，丛枝菌根真菌(Funneliformis Mosseae)对青杨形态、生理和修复能力的影响。与外来AMF共生对两性的生长和生物量分配没有显著影响。然而，接种丛枝菌根真菌后，雌雄在根中吸收了更多的P，但不吸收N，尤其是在外源添加铅的情况下。在这种条件下，营养状况的改善可能与抗氧化酶(特别是超氧化物歧化酶(SOD)和过氧化氢酶(CAT))活性的进一步提高以及对过量活性氧(ROS)诱导的氧化胁迫的缓解有关。我们还观察到，外源AMF能够促进雌性根部对铅的吸收和积累，但对雄性根部铅的吸收和积累没有促进作用。因此，在这种扩散污染水平下，受感染的雌性可能比受感染的雄性更适合修复这种金属，因为雌性比受感染的雄性具有更高的HM积累能力，而对生长和生理特性没有明显的负面影响。此外，由于土壤微生物群落的多样性及其相互作用的复杂性，需要实地调查来验证我们的实验结果。(C)2014 Elsevier Inc.保留所有权利。
Using fast-growing trees to remediate soils polluted by heavy metals (HMs) has received increasingly more attention, especially for recalcitrant Pb, as one of the most seriously toxic HMs. However, little is known about the responses of plants to a diffused level of Pb pollution, and a more combined phytor-emediation technique is needed to explore. In this study, an arbuscular mycorrhizal fungus (AMF), i.e., Funneliformis mosseae, isolated from Populus euphratica distributed in a tailing of Pb/Zn ore, was introduced to investigate its effects on sex-specific responses of P. cathayana in morphology, physiology, and Pb phytoremediation capacity, when exposed to a diffused level of Pb pollution (100 mg Pb2+ kg(-1) dry soil). Symbiosis with exotic AMF did not significantly affect growth of both sexes and biomass allocation. However, when inoculated with AMF, both sexes absorbed more P, but not N in the roots, especially when exposed to the exogenous addition of Pb. The improvement of nutrient status under such conditions might be associated with a further increase in activity of antioxidant enzymes (particularly for superoxide dismutase (SOD) and catalase (CAT)), and the mitigation of oxidation stress induced by excessive reactive oxygen species (ROS). We also observed that exotic AMF could promote the uptake and accumulation of Pb in roots of females, but not in that of males. Therefore, under this diffused pollution level, the infected females might be more suitable for remediation of this metal than infected males, due to the higher capacity of HM accumulation without obvious negative effects on growth and physiological traits. Moreover, field surveys are needed to testify our experimental results, due to diversity of soil microbial community and complexities of their interaction. (C) 2014 Elsevier Inc. All rights reserved.