TRANSCRIPTION TERMINATION OCCURS WITHIN A 1000 BASE PAIR REGION DOWNSTREAM FROM THE POLY(A) SITE OF THE MOUSE BETA-GLOBIN (MAJOR) GENE)
TRANSCRIPTION TERMINATION OCCURS WITHIN A 1000 BASE PAIR REGION DOWNSTREAM FROM THE POLY(A) SITE OF THE MOUSE BETA-GLOBIN (MAJOR) GENE)
复制标题
DOI：
10.1093/nar/12.22.8723
复制
发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
DARNELL, JE
中科院分区：
文献类型：
--
作者：
CITRON, B;FALCKPEDERSEN, E;DARNELL, JE
从诱导的小鼠红白血病细胞的细胞核中，β-珠蛋白(主要)基因的转录终止于聚(A)加成部位下游的700到2000个碱基区域，但不是在特别受欢迎的部位。此外，我们首次对来自细胞转录单位的病毒标记的RNA进行了分析，该分析表明RNA终止于通过分析玻璃标记的RNA而诊断的相同区域。终止发生的区域包含在从聚(A)位点下游600个碱基开始测序的1414个碱基中。序列AATAAA在开始处的频率增加，并且在该区域的末端附近有茎和环结构以及紧随其后的一串TS。
Transcription of RNA from the β-globin (major) gene in nuclei from induced mouse erythroleukemia cells terminates within the region between 700 to 2000 bases downstream from the poly (A) addition site, but not at particularly favored sites. In addition we present the first analysis ofin vivolabeled RNA from a cell transcription unit that shows RNA termination in the same region as diagnosed by analysis ofin vitrolabeled RNA. The region in which termination occurs is contained in 1414 bp that were sequenced beginning 600 bases downstream from the poly(A) site. There is an increased frequency of the sequence AATAAA at the beginning and a stem and loop structure followed by a string of Ts near the end of this region.