Simultaneous determination of cefepime and vancomycin in plasma and cerebrospinal fluid by MEKC with direct sample injection and application for bacterial meningitis
Simultaneous determination of cefepime and vancomycin in plasma and cerebrospinal fluid by MEKC with direct sample injection and application for bacterial meningitis
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DOI：
10.1002/elps.200600537
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发表时间：
2007-06-01
期刊：
影响因子：
2.9
通讯作者：
Chen, Su-Hwei
中科院分区：
文献类型：
--
作者：
Yang, Yuan-Han;Wu, Wen-Yi;Chen, Su-Hwei
本文介绍了一种简单的毛细管电泳-紫外检测器，在214 nm波长处直接进样同时测定血浆和脑脊液中头孢吡肟和万古霉素的方法。在25 ℃下使用由含有SDS的Tris缓冲液和甲醇作为电解质溶液组成的BGE从生物基质中分离头孢吡肟和万古霉素。在优化的胶束电动色谱条件下，生物样品的分离效果好，分析效率高，分析时间短。研究了影响药物从生物基质中分离的几个参数，包括甲醇、pH、Tris缓冲液和SDS的浓度。分别以咪唑或头孢唑林为内标，测定血浆和脑脊液中头孢吡肟和万古霉素的线性范围均为1-30 μ g/mL;生物基质中头孢吡肟和万古霉素的检测限（进样电压16 kV，15 s）分别为0.3和0.5 μ g/mL。所提出的方法用于测定脑膜炎患者静脉给药后采集的血浆和CSF中头孢吡肟和万古霉素的适用性得到了证明。
A simple MEKC with UV detection at 214 nm for simultaneous analysis of cefepime and vancomycin in plasma and in cerebrospinal fluid (CSF) by direct injection without any sample pretreatment is described. The separation of cefepime and vanconrycin from biological matrices was performed at 25 degrees C using a BGE consisting of a Tris buffer with SDS and methanol as the electrolyte solution. Under optimal MEKC conditions for biological samples, good separations with high efficiency and short analysis time are achieved. Several parameters affecting the separation of the drugs from biological matrices were studied, including methanol, pH, and concentrations of the Tris buffer and SDS. The linear ranges of the method for the determination of cefepime and vancomycin in plasma and in CSF using imidazole or cefazolin as an internal standard, respectively, were all cover the range of 1-30 mu g/mL; the detection limits of cefepime and vancomycin in biological matrices (injection 16 kV, 15 s) were 0.3 and 0.5 mu g/mL, respectively. The applicability of the proposed method for the determination of cefepime and vancomycin in plasma and CSF collected after intravenous administration of the drugs in patients with meningitis was demonstrated.