Behavioral and omics analyses study on potential involvement of dipeptide balenine through supplementation in diet of senescence-accelerated mouse prone 8.
Behavioral and omics analyses study on potential involvement of dipeptide balenine through supplementation in diet of senescence-accelerated mouse prone 8.
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DOI：
10.1016/j.gdata.2016.09.004
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发表时间：
2016-12
期刊：
影响因子：
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通讯作者：
Shioda, Seiji
中科院分区：
文献类型：
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作者：
Wada, Nobuhiro;Yamanaka, Satoru;Shibato, Junko;Rakwal, Randeep;Hirako, Satoshi;Iizuka, Yuzuru;Kim, Hyounju;Matsumoto, Akiyo;Kimura, Ai;Takenoya, Fumiko;Yasunaga, Genta;Shioda, Seiji
本研究调查了作为鲸肉提取物（以下简称 WME）主要成分的二肽鲸鱼碱，对加速衰老的小鼠 pron 8 (SAMP8)、阿尔茨海默病 (AD) 模型在学习和记忆形成水平以及大脑全基因组表达谱方面的影响。喂食实验性巴林宁 (+ WME) 补充饮食 26 周的小鼠接受了四项行为测试——开放场地、Y 迷宫、新物体识别和充满水的多重 T 迷宫——以检查对学习和记忆的影响。在 4 × 44 K 小鼠全基因组 DNA 微阵列芯片上描绘了喂食 WME 饮食的 SAMP8 小鼠与对照低红花油 (LSO) 饮食喂养的小鼠的脑转录组。结果显示，WME 饮食不仅能改善 SAMP8 小鼠的学习和记忆形成，还能积极调节大脑的变化；基因清单可供科学界公开分析。有趣的是，SAMP8小鼠模型呈现出AD的许多遗传特征，并且在SAMR1（衰老加速小鼠抗性1）小鼠上发现了许多新分子（Slc2a5、Treh、Fbp1、Aldob、Ppp1r1a、DNase1、Agxt2l1、Cyp2e1、Acsm1、Acsm2和Pah），这些分子在balenine的作用下受到相反的调节/恢复(+ WME) 通过 DNA 微阵列和生物信息学分析补充饮食方案。我们目前的研究展示了一种实验策略，用于了解肉类饮食中主要含有的二肽 balenine 对 SAMP8 的影响，为全基因组范围内的全脑转录组变化提供新的见解。基因表达数据已存入基因表达综合库 (GEO)：GSE76459。这些数据将成为检查天然产品效果的宝贵资源，也可以作为进一步功能分析和研究的人体模型。
This study investigates effects of dipeptide balenine, as a major component of whale meat extract (hereafter, WME), supplementation on senescence-accelerated mouse prone 8 (SAMP8), an Alzheimer's disease (AD) model at level of learning and memory formation and brain expression profiles genome-wide in brain. Mice fed experimental balenine (+ WME) supplemented diet for 26 weeks were subjected to four behavioral tests – open field, Y-maze, novel object recognition, and water-filled multiple T-maze – to examine effects on learning and memory. Brain transcriptome of SAMP8 mice-fed the WME diet over control low-safflower oil (LSO) diet-fed mice was delineated on a 4 × 44 K mouse whole genome DNA microarray chip. Results revealed the WME diet not only induced improvements in the learning and memory formation but also positively modulated changes in the brain of the SAMP8 mouse; the gene inventories are publically available for analysis by the scientific community. Interestingly, the SAMP8 mouse model presented many genetic characteristics of AD, and numerous novel molecules (Slc2a5, Treh, Fbp1, Aldob, Ppp1r1a, DNase1, Agxt2l1, Cyp2e1, Acsm1, Acsm2, and Pah) were revealed over the SAMR1 (senescence-accelerated mouse resistant 1) mouse, to be oppositely regulated/recovered under the balenine (+ WME) supplemented diet regime by DNA microarray and bioinformatics analyses. Our present study demonstrates an experimental strategy to understand the effects of dipeptide balenine, prominetly contained in meat diet, on SAMP8, providing new insight into whole brain transcriptome changes genome-wide. The gene expression data has been deposited into the Gene Expression Omnibus (GEO): GSE76459. The data will be a valuable resource in examining the effects of natural products, and which could also serve as a human model for further functional analysis and investigation.