Transport governs flow-enhanced cell tethering through L-selectin at threshold shear.
Transport governs flow-enhanced cell tethering through L-selectin at threshold shear.
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DOI：
10.1529/biophysj.106.090969
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发表时间：
2007
影响因子：
3.4
通讯作者：
T. Yago;V. Zarnitsyna;A. Kl̶opocki;R. McEver;C. Zhu
中科院分区：
文献类型：
--
作者：
T. Yago;V. Zarnitsyna;A. Kl̶opocki;R. McEver;C. Zhu
Flow-enhanced cell adhesion is a counterintuitive phenomenon that has been observed in several biological systems. Flow augments L-selectin-dependent adhesion by increasing the initial tethering of leukocytes to vascular surfaces and by strengthening their subsequent rolling interactions. Tethering or rolling might be influenced by physical factors that affect the formation or dissociation of selectin-ligand bonds. We recently demonstrated that flow enhanced rolling of L-selectin-bearing microspheres or neutrophils on P-selectin glycoprotein ligand-1 by force decreased bond dissociation. Here, we show that flow augmented tethering of these microspheres or cells to P-selectin glycoprotein ligand-1 by three transport mechanisms that increased bond formation: sliding of the sphere bottom on the surface, Brownian motion, and molecular diffusion. These results elucidate the mechanisms for flow-enhanced tethering through L-selectin.
