HYDRATED AUTOCLAVE PRETREATMENT ENHANCEMENT OF PRION PROTEIN IMMUNOREACTIVITY IN FORMALIN-FIXED BOVINE SPONGIFORM ENCEPHALOPATHY-AFFECTED BRAIN
HYDRATED AUTOCLAVE PRETREATMENT ENHANCEMENT OF PRION PROTEIN IMMUNOREACTIVITY IN FORMALIN-FIXED BOVINE SPONGIFORM ENCEPHALOPATHY-AFFECTED BRAIN
复制标题
DOI：
10.1007/bf00386258
复制
发表时间：
1994-01-01
影响因子：
12.7
通讯作者：
WELLS, GAH
中科院分区：
文献类型：
--
作者：
HARITANI, M;SPENCER, YI;WELLS, GAH
应用自动图像分析技术对福尔马林固定、石蜡包埋的牛海绵状脑病(BSE)脑组织中PrP的3种检测方法进行了比较。在水蒸气灭菌处理30min的切片中，以颗粒表达的形式最为丰富。甲酸处理的切片免疫染色较少，而水解式高压灭菌法处理的切片未检测到特殊颗粒免疫染色。在PrP免疫标记之前对切片进行水合高压灭菌预处理，可以可视化大脑中异常PrP沉积的广泛位置，从而能够详细研究受BSE影响的常规固定的牛中枢神经系统中蛋白质的形式和分布。
The efficacy of three pretreatment techniques for the detection of prion protein (PrP) in formalin-fixed, paraffin-embedded bovine spongiform encephalopathy (BSE) - affected brain tissue were compared using automated image analysis. The most abundant immunostaining was in the form of particulate expression observed in sections pretreated with hydrated autoclaving for 30 min. Considerably less immunostaining occurred in sections pretreated with formic acid and no specific particulate immunostaining was detected in sections pretreated with hydrolytic autoclaving. Hydrated autoclaving pretreatment of sections prior to PrP immunolabelling gives visualisation of widespread sites of abnormal PrP deposition in the brain, allowing detailed study of the form and distribution of the protein in routinely fixed bovine central nervous system affected with BSE.