ENTAMOEBA-INVADENS AND E-HISTOLYTICA - SEPARATION AND PURIFICATION OF PRECYSTS AND CYSTS BY CENTRIFUGATION ON DISCONTINUOUS DENSITY GRADIENTS OF PERCOLL
ENTAMOEBA-INVADENS AND E-HISTOLYTICA - SEPARATION AND PURIFICATION OF PRECYSTS AND CYSTS BY CENTRIFUGATION ON DISCONTINUOUS DENSITY GRADIENTS OF PERCOLL
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DOI：
10.1016/0014-4894(83)90022-x
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发表时间：
1983-01-01
影响因子：
2.1
通讯作者：
MIRELMAN, D
中科院分区：
文献类型：
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作者：
AVRON, B;BRACHA, R;MIRELMAN, D
人类肠道寄生虫E.在聚乙烯吡咯烷酮包被的胶态二氧化硅颗粒（Percoll）的密度阶跃梯度上快速和定量地分离侵袭虫（滋养体、前囊和囊）。用此方法研究了E.可以监测侵入体滋养体。Percoll梯度也能有效分离E.溶组织菌和细菌[福氏志贺菌和金黄色葡萄球菌]。在Percoll上纯化后，滋养体在通过光学显微镜检查时没有显示出损伤的证据，并且根据其繁殖能力判断没有活力损失。
The different cell forms in the life cycle of [the human intestinal parasite] E. invadens (trophozoites, precysts and cysts) were rapidly and quantitatively separated on density step gradients of polyvinylpyrolidone-coated colloidal silica particles (Percoll). With this method, the gradual process of encystation by E. invadens trophozoites could be monitored. Percoll gradients were also efficient in separating trophozoites of E. histolytica and bacteria [Shigella flexneri and Staphylococcus aurens]. After purification on Percoll, trophozoites display no evidence of damage when examined by light microscopy and no loss in viability as judged by their ability to multiply.