ApoA-II Directs Morphogenetic Movements of Zebrafish Embryo by Preventing Chromosome Fusion during Nuclear Division in Yolk Syncytial Layer*
ApoA-II Directs Morphogenetic Movements of Zebrafish Embryo by Preventing Chromosome Fusion during Nuclear Division in Yolk Syncytial Layer*
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DOI：
10.1074/jbc.m110.134908
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发表时间：
2011-01
期刊：
影响因子：
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通讯作者：
Ting Zhang;Shaohua Yao;Ping Wang;Chaoran Yin;Chun Xiao;Meilin Qian;Donghui Liu;Lemin Zheng;W. Meng;Hongyang Zhu;Jin Liu;Hong Xu;X. Mo
中科院分区：
文献类型：
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作者：
Ting Zhang;Shaohua Yao;Ping Wang;Chaoran Yin;Chun Xiao;Meilin Qian;Donghui Liu;Lemin Zheng;W. Meng;Hongyang Zhu;Jin Liu;Hong Xu;X. Mo
高密度脂蛋白（HDL）是一类含有脂质和蛋白质的颗粒，由两种主要载脂蛋白apoA-I和apoA-II组成。ApoA-II已被证明参与胰岛素抵抗、肥胖、糖尿病和代谢综合征的发病机制。在胚胎中，apoa2 mrna在肝脏、脑、肺、胎盘和鱼黄合胞层（YSL）中大量存在，表明apoa2可能在胚胎发育过程中发挥作用。我们发现apoa2通过调节YSL的组织来调节斑马鱼的胚胎发育。斑马鱼体内apoa2功能的破坏导致染色体融合，强烈阻断YSL核分裂，诱发YSL组织紊乱，最终扰乱胚胎表观代谢。纯化的人原生apoA-II能够特异性地修复斑马鱼胚胎和人HeLa细胞的缺陷并诱导核分裂。在斑马鱼胚胎和人HeLa细胞的YSL核分裂过程中，apoA-II的C端是染色体正确分离所必需的。我们的数据表明，YSL的组织是囊胚形成和形态发生所必需的，并表明载脂蛋白apoA-II是YSL核分裂的一个新因子，参与调控斑马鱼早期胚胎形态发生和哺乳动物细胞增殖。
The high density lipoprotein (HDL) represents a class of lipid- and protein-containing particles and consists of two major apolipoproteins apoA-I and apoA-II. ApoA-II has been shown to be involved in the pathogenesis of insulin resistance, adiposity, diabetes, and metabolic syndrome. In embryo, apoa2 mRNAs are abundant in the liver, brain, lung, placenta, and in fish yolk syncytial layer (YSL), suggesting that apoa2 may perform a function during embryonic development. Here we find out that apoa2 modulates zebrafish embryonic development by regulating the organization of YSL. Disruption of apoa2 function in zebrafish caused chromosome fusing, which strongly blocked YSL nuclear division, inducing disorders in YSL organization and finally disturbing the embryonic epiboly. Purified native human apoA-II was able specifically to rescue the defects and induced nuclear division in zebrafish embryos and in human HeLa cells. The C terminus of apoA-II was required for the proper chromosome separation during nuclear division of YSL in zebrafish embryos and in human HeLa cells. Our data indicate that organization of YSL is required for blastoderm patterning and morphogenesis and suggest that apolipoprotein apoA-II is a novel factor of nuclear division in YSL involved in the regulation of early zebrafish embryonic morphogenesis and in mammalian cells for proliferation.