Use of a combined cryo-EM and X-ray crystallography approach to reveal molecular details of bacterial pilus assembly by the chaperone/usher pathway.
Use of a combined cryo-EM and X-ray crystallography approach to reveal molecular details of bacterial pilus assembly by the chaperone/usher pathway.
复制标题
使用冷冻电镜和 X 射线晶体学相结合的方法揭示分子伴侣/引座途径细菌菌毛组装的分子细节。
DOI：
10.1016/j.mib.2009.03.002
复制
发表时间：
2009
影响因子：
5.4
通讯作者：
Thanassi,DavidG
中科院分区：
文献类型：
--
作者：
Li,Huilin;Thanassi,DavidG
许多细菌在其细胞表面组装称为皮利或菌毛的毛发状纤维。这些纤维介导粘附到各种表面，包括宿主细胞，并在发病机制中发挥关键作用。皮利是由数千个单独的亚单位蛋白质组成的聚合物。了解这些亚基蛋白如何穿过细菌包膜并在细胞表面正确组装不仅对基础生物学而且对新型抗菌剂的开发都很重要。伴侣/usher pilus生物合成途径是最好理解的蛋白质分泌系统之一，这在很大程度上要归功于生物物理技术的创新努力，如X射线晶体学和冷冻电子显微镜。这种结合的方法有望进一步阐明关于多步骤和高度动态的菌毛组装过程的剩余问题，以及研究其他蛋白质分泌和细胞器生物合成系统。
Many bacteria assemble hair-like fibers termed pili or fimbriae on their cell surface. These fibers mediate adhesion to various surfaces, including host cells, and play crucial roles in pathogenesis. Pili are polymers composed of thousands of individual subunit proteins. Understanding how these subunit proteins cross the bacterial envelope and correctly assemble at the cell surface is important not only for basic biology but also for the development of novel antimicrobial agents. The chaperone/usher pilus biogenesis pathway is one of the best-understood protein secretion systems, thanks largely to innovative efforts in biophysical techniques such as X-ray crystallography and cryo-electron microscopy. Such a combined approach holds promise for further elucidating remaining questions regarding the multi-step and highly dynamic pilus assembly process, as well as for studying other protein secretion and organelle biogenesis systems.