Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades
Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades
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DOI：
10.1111/j.1750-2659.2010.00177.x
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发表时间：
2011-01-01
影响因子：
4.4
通讯作者：
Fomsgaard, Anders
中科院分区：
文献类型：
--
作者：
Bragstad, Karoline;Martel, Cyril J.;Fomsgaard, Anders
背景由于传统的蛋白质疫苗不能诱导广泛的交叉反应性，因此需要替代的流感疫苗和疫苗生产形式。此外，快速疫苗生产在大流行情况下尤其重要，并且更广泛的疫苗反应性将减少频繁改变疫苗制剂的需要。我们比较了大流行性流感DNA疫苗诱导针对亚型内远亲毒株的免疫力的能力与常规三价蛋白疫苗诱导的针对同源病毒攻击的免疫力。基于1918 H1N1西班牙流感大流行病毒或1968 H3 N2香港流感大流行病毒的血凝素（HA）和神经氨酸酶（NA）和/或基质（M）和核蛋白基因的DNA疫苗的颗粒介导的表皮递送（基因枪）。当代H1N1或H3 N2病毒的动物进行了挑战。ResultsWe表明，DNA疫苗编码的蛋白质的原始1918年H1N1大流行性病毒诱导保护性交叉反应免疫反应的雪貂对感染1947年H1N1病毒和最近的1999年H1N1病毒。同样，DNA疫苗，基于HA和NA的1968年H3 N2大流行病毒，诱导交叉反应性免疫应答对最近的2005年H3 N2病毒challenge.ConclusionsDNA疫苗基于大流行或最近的季节性流感基因诱导交叉反应性免疫对当代病毒的挑战一样好或上级当代传统的三价蛋白疫苗。这表明流感DNA具有独特的能力，可以诱导针对当代和长期漂移病毒的交叉保护性免疫。
BackgroundAlternative influenza vaccines and vaccine production forms are needed as the conventional protein vaccines do not induce broad cross-reactivity against drifted strains. Furthermore, fast vaccine production is especially important in a pandemic situation, and broader vaccine reactivity would diminish the need for frequent change in the vaccine formulations.ObjectiveIn this study, we compared the ability of pandemic influenza DNA vaccines to induce immunity against distantly related strains within a subtype with the immunity induced by conventional trivalent protein vaccines against homologous virus challenge.MethodsFerrets were immunised by particle-mediated epidermal delivery (gene gun) with DNA vaccines based on the haemagglutinin (HA) and neuraminidase (NA) and/or the matrix (M) and nucleoprotein genes of the 1918 H1N1 Spanish influenza pandemic virus or the 1968 H3N2 Hong Kong influenza pandemic virus. The animals were challenged with contemporary H1N1 or H3N2 viruses.ResultsWe demonstrated that DNA vaccines encoding proteins of the original 1918 H1N1 pandemic virus induced protective cross-reactive immune responses in ferrets against infection with a 1947 H1N1 virus and a recent 1999 H1N1 virus. Similarly, a DNA vaccine, based on the HA and NA of the 1968 H3N2 pandemic virus, induced cross-reactive immune responses against a recent 2005 H3N2 virus challenge.ConclusionsDNA vaccines based on pandemic or recent seasonal influenza genes induced cross-reactive immunity against contemporary virus challenge as good as or superior to contemporary conventional trivalent protein vaccines. This suggests a unique ability of influenza DNA to induce cross-protective immunity against both contemporary and long-time drifted viruses.