Fluorescence location of RVLM kainate microinjections that alter the control of breathing.
Fluorescence location of RVLM kainate microinjections that alter the control of breathing.
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改变呼吸控制的 RVLM 红藻氨酸显微注射的荧光位置。
DOI：
10.1152/jappl.1990.68.3.1157
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Li,AH
中科院分区：
文献类型：
--
作者：
Nattie,EE;Li,AH
在氯醛糖-尿烷麻醉、迷走神经切断、麻痹、肾小球切除、伺服通气的猫中，将红藻氨酸 (4.7 mM) 施用于延髓头端腹外侧 (RVLM) 表面可降低膈输出、CO2 敏感性和血压。在本研究中，使用相同的制剂，在 RVLM 表面下方双侧注射 50 至 100 nl 红藻氨酸，更好地定位这些反应的地形。然后将单侧 10-nl 红藻氨酸注射的生理反应与荧光微珠（直径 0.5 微米）确定的解剖位置相关联。许多部位没有影响，少数头侧和尾侧部位膈活动增加，膈活动减少的一组部位经常导致呼吸暂停、CO2 敏感性降低以及对颈动脉窦神经刺激的反应降低。血压未受影响。这些位点距离表面 400 微米以内，位于面核的腹侧、副巨细胞外侧核的腹外侧、上橄榄的尾侧以及面后核的喙侧。它们似乎位于最近描述的梯形后核内，该核包含具有呼吸相关活动的细胞以及向背侧和腹侧呼吸群的投射。该位点内的细胞似乎能够为呼吸提供补给输入并影响外周和中枢化学感受。
Kainic acid (4.7 mM) applied to the rostral ventrolateral medulla (RVLM) surface decreases phrenic output, CO2 sensitivity, and blood pressure in chloralose-urethan-anesthetized, vagotomized, paralyzed, glomectomized, servoventilated cats. In this study using the same preparation, bilateral 50- to 100-nl kainate injections just below the RVLM surface better localized these responses topographically. The physiological responses to unilateral 10-nl kainate injections were then correlated with anatomic location determined by fluorescent microbeads (0.5 micron diam). Many sites were associated with no effect, a few rostral and caudal sites with increased phrenic activity, and cluster of sites with decreased phrenic activity often to apnea, decreased CO2 sensitivity, and decreased responses to carotid sinus nerve stimulation. Blood pressure was unaffected. These sites, within 400 microns of the surface, were ventral to the facial nucleus, ventrolateral to the nucleus paragigantocellularis lateralis, caudal to the superior olive, and rostral to the retrofacial nucleus. They appeared to be within the recently described retrotrapezoid nucleus, which contains cells with respiratory-related activity and projections to the dorsal and ventral respiratory groups. Cells within this site appear able to provide tonic input to respiration and to affect peripheral and central chemoreception.