Separation of furostanol saponins by supercritical fluidchromatography
Separation of furostanol saponins by supercritical fluidchromatography
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DOI：
10.1016/j.jpba.2017.05.023
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发表时间：
2017
影响因子：
3.4
通讯作者：
mabaiping
中科院分区：
文献类型：
--
作者：
yang jie;zhu lingling;zhaoyang;xu yongwei;sun qinglong;liu shuchen;liuchao;mabaiping
Supercritical fluid chromatography (SFC) has good separation efficiency and is suitable for separatingweakly polar compounds. Furostanol saponins, as an important kind of steroidal saponins, generallyhave two sugar chains, which are polar and hydrophilic. The hydroxyl group at the C-22 position offurostanol saponins is active and easily reacts with lower alcohols under appropriate conditions. Theseparation of hydrophilic furostanol saponins was tested by SFC in this study. The effects of chromato-graphic conditions on the separation of the mixed furostanol saponins and their hydroxyl derivativesat the C-22 position were studied. The conditions for SFC, which included different column polarity,modifier, additive, and column temperature, were tested. After optimization, the mixed 10 similar struc-tures of furostanol saponins were separated in 22 min on the Diol column at a temperature of 40◦C. Themobile phase was CO2(mobile phase A) and methanol (containing 0.2% NH3·H2O and 3% H2O) (mobilephase B). The backpressure was maintained isobarically at 11.03 MPa. SFC was found to be effectivein separating the furostanol saponins that shared the same aglycone but varied in sugar chains. SFCwas sensitive to the number and type of sugars. The resolution of furostanol saponin isomers was notideal. The extract of Dioscorea zingiberensis C. H. Wright was profiled by SFC–quadrupole time-of-flightmass spectrometry. The main saponins of the extract were well separated. Therefore, SFC could be usedfor separating hydrophilic furostanol saponins and analyzing traditional Chinese medicines that mainlycontained steroidal saponins.
