URINARY-EXCRETION OF UNMETABOLIZED BENZENE AS AN INDICATOR OF BENZENE EXPOSURE
URINARY-EXCRETION OF UNMETABOLIZED BENZENE AS AN INDICATOR OF BENZENE EXPOSURE
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DOI：
10.1080/15287399309531715
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发表时间：
1993-03-01
期刊：
影响因子：
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通讯作者：
IMBRIANI, M
中科院分区：
文献类型：
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作者：
GHITTORI, S;FIORENTINO, ML;IMBRIANI, M
通过将尿液上清液注入气相色谱仪来测定 110 名在化工厂和汽油泵中接触苯的工人的尿样中的苯浓度（C(u)，ng/L）。尿液中充满无水 N2SO4，以促进空气中的苯通过尿液。将溶剂从尿液表面汽提并通过抽吸泵（流速150ml/m）浓缩在吸附剂基底（Carbotrap管）上。通过同时通过木炭吸入过滤空气来实现顶部空间的清洗。苯被热解吸并注入柱中（热管解吸器，Supelco；370℃热闪蒸；硼硅酸盐毛细管玻璃柱SPB-1，60m长，0.75mm内径，1μm膜厚；GC Dani 8580-FID）。还测定了 40 名非暴露受试者（20 名吸烟者 > 20 支/天和 20 名非吸烟者）尿液中的苯浓度 [中位值分别为 790 ng/L (10. 17 nmol/L) 和 131 ng/L (1. 70 nmol/L)]。通过木炭管监测个体工人的 8 小时时间加权暴露强度（C(l), mug/m3）。苯暴露的中值为 736 mug/m3 (9.42 mumol/m3) [几何标准差 (GSD) = 2.99；范围 64 微摩尔/立方米 (0.82 微摩尔/立方米) 至 13,387 微摩尔/立方米) (171.30 微摩尔/立方米)]。尿液中苯浓度 (C(u), ng/L) 和呼吸区苯浓度 (C(l), mug/m3) 之间存在以下线性相关性： log(C(u)) = 0.645 x log(C(l)) + 1.399 r = .559, n = 110, p < .0001 在研究中排除吸烟的工人后，空气苯浓度与苯之间的相关性尿液中测得的苯含量为： log(C(u)) = 0.872 x log(C(l)) + 0.6 r = .763, n = 63, p < .0001 研究结果表明，尿液苯含量是职业接触苯的指标。
Benzene concentrations in urine samples (C(u), ng/L) from 110 workers exposed to benzene in chemical plants and gasoline pumps were determined by injecting urine supernate into a gas chromatograph. The urine was saturated with anhydrous N2SO4 to facilitate the passage of benzene in the air over the urine. The solvent was stripped from the urine surface and concentrated on an adsorbent substrate (Carbotrap tube) by means of a suction pump (flow rate 150 ml/m). Wash-up of the head space was achieved by simultaneous intake of filtered air through charcoal Benzene was thermically desorbed and injected in a column (thermal tube desorber, Supelco; 370-degrees-C thermal flash; borosilicate capillary glass column SPB-1, 60 m length, 0.75 mm ID, 1 mum film thickness; GC Dani 8580-FID). Benzene concentrations in the urine from 40 non-exposed subjects (20 smokers > 20 cigarettes/d and 20 nonsmokers) were also determined [median value of 790 ng/L (10. 17 nmol/L) and 131 ng/L (1. 70 nmol/L), respectively]. The 8-h time-weighted exposure intensity (C(l), mug/m3) of individual workers was monitored by means of charcoal tubes. The median value for exposure to benzene was 736 mug/m3 (9.42 mumol/m3) [geometric standard deviation (GSD) = 2.99; range 64 mug/m3 (0.82 mumol/m3) to 13,387 mug/m3) (171.30 mumol/m3)]. The following linear correlation was found between benzene concentrations in urine (C(u), ng/L) and benzene concentrations in the breathing zone (C(l), mug/m3): log(C(u)) = 0.645 x log(C(l)) + 1.399 r = .559, n = 110, p < .0001 With exclusion of workers who smoked from the study, the correlation between air benzene concentration and benzene measured in urine was: log(C(u)) = 0.872 x log(C(l)) + 0.6 r = .763, n = 63, p < .0001 The study results indicate that the urinary level of benzene is an indicator of occupational exposure to benzene.