Dorsal root ganglion neurons with dichotomizing axons projecting to the hip joint and the knee skin in rats: possible mechanism of referred knee pain in hip joint disease
Dorsal root ganglion neurons with dichotomizing axons projecting to the hip joint and the knee skin in rats: possible mechanism of referred knee pain in hip joint disease
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DOI：
10.1007/s00776-011-0144-1
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发表时间：
2011-11-01
影响因子：
1.7
通讯作者：
Takahashi, Kazuhisa
中科院分区：
文献类型：
--
作者：
Miura, Yoko;Ohtori, Seiji;Takahashi, Kazuhisa
患有髋关节疾病的患者有时会抱怨膝盖疼痛以及髋关节疼痛。然而，髋关节和膝关节的感觉神经的精确感觉神经支配模式和相关性尚不清楚。本研究采用荧光双标记技术观察了大鼠背根神经节（DRG）内向髋关节和膝关节皮肤投射的二叉轴突神经元，并采用降钙素基因相关肽（CGRP）等炎性神经肽免疫组织化学染色方法观察了二叉轴突神经元的特征。荧光金（FG）和1，1 '-双十八烷基-3，3，3'，3 '-四甲基吲哚羰花青高氯酸盐（DiI）用于双标记研究。将FG注射到左髋关节中，并将DiI应用于膝盖皮肤的左内侧部分。10 d后取双侧背根神经节进行CGRP免疫组化染色，仅左侧L2 ~ L4可见FG和DiI双标神经元。约1.6%的支配髋关节的DRG神经元有其他轴突延伸到膝关节皮肤的内侧部分，35%的双标记神经元为CGRP阳性。CGRP阳性神经元可能在牵涉性膝关节痛伴髋关节痛的表现中起一定作用。
Patients who have hip joint diseases sometimes complain of knee pain as well as hip joint area pain. However, the precise sensory innervation pattern and correlation of the sensory nerves of the hip joint and knee are unknown. The purpose of this study was to investigate dorsal root ganglion (DRG) neurons with dichotomizing axons projecting to both the hip joint and the knee skin in rats using double fluorescent labeling techniques, and to examine characteristics of the DRG neurons with dichotomizing axons using immunohistochemical staining for inflammatory neuropeptides such as calcitonin gene-related peptide (CGRP).For 20 rats, two kinds of neurotracers, Fluoro-Gold (FG) and 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI), were used in the double-labeling study. FG was injected into the left hip joint, and DiI was applied to the left medial portion of knee skin. Ten days after application, bilateral DRGs were harvested and immunohistochemically stained for CGRP.DRG neurons double labeled with FG and DiI were observed only from L2 to L4 on the left side. Approximately 1.6% of all DRG neurons innervating the hip joints had other axons that extended to the medial portion of knee skin, and 35% of double-labeled neurons were CGRP positive.Our results showed that the double-labeled neurons had peripheral axons that dichotomized into both the hip joint and the knee skin. CGRP-positive neurons of these dichotomizing fibers may play some role in the manifestation of referred knee pain with hip joint pain.