Human snRNP polypeptide D1 promotes pre-mRNA splicing in yeast and defines nonessential yeast Smd1p sequences.
Human snRNP polypeptide D1 promotes pre-mRNA splicing in yeast and defines nonessential yeast Smd1p sequences.
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DOI：
10.1093/nar/21.15.3501
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发表时间：
1993-07
影响因子：
14.9
通讯作者：
Brian C. Rymond;L. Rokeach;S. Hoch
中科院分区：
文献类型：
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作者：
Brian C. Rymond;L. Rokeach;S. Hoch
对酵母和后生动物前 mRNA 剪接的平行研究已经证明了细胞剪接装置（剪接体）的核酸和蛋白质成分的巨大复杂性。酵母和后生动物剪接体蛋白在组成和结构上的差异程度目前尚不清楚。在本报告中，我们证明人类小核核糖核蛋白 (snRNP) 多肽 D1 可以补充与酵母 smd1 无效等位基因相关的细胞致死性、剪接缺陷和 snRNA 不稳定表型。通过比较预测的酵母蛋白和人类蛋白，对酵母 SMD1 进行突变分析，结果表明 Smd1p 的很大一部分非保守部分对于生物活性来说是可有可无的。这些观察结果牢固地确立了 D1 作为细胞剪接装置的重要组成部分，并表明酵母和后生动物在指导早期 snRNP 组装的多肽方面非常相似。
Parallel investigations of yeast and metazoan pre-mRNA splicing have documented enormous complexity in the nucleic acid and protein components of the cellular splicing apparatus, the spliceosome. The degree to which yeast and metazoan spliceosomal proteins differ in composition and structure is currently unknown. In this report we demonstrate that the human small nuclear ribonucleoprotein (snRNP) polypeptide D1 complements the cell lethality, splicing deficiency, and snRNA instability phenotypes associated with a yeast smd1 null allele. Mutational analysis of yeast SMD1, guided by a comparison of the predicted yeast and human proteins, reveals that a large, nonconserved portion of Smd1p is dispensable for biological activity. These observations firmly establish D1 as an essential component of the cellular splicing apparatus and suggest that yeast and metazoa are remarkably similar in the polypeptides guiding early snRNP assembly.