Physiological and molecular responses of the earthworm (Eisenia fetida) to soil chlortetracycline contamination
Physiological and molecular responses of the earthworm (Eisenia fetida) to soil chlortetracycline contamination
复制标题
蚯蚓（Eisenia fetida）对土壤金霉素污染的生理和分子反应
DOI：
10.1016/j.envpol.2012.07.020
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发表时间：
2012-12-01
影响因子：
8.9
通讯作者：
Li, Ye
中科院分区：
文献类型：
--
作者：
Lin, Dasong;Zhou, Qixing;Li, Ye
本研究旨在评估土壤中暴露于氯四环素（CTC）对蚯蚓生殖终点（幼虫数和茧数）、生化反应和遗传毒性潜力的毒性影响。结果表明，暴露于CTC后，蚯蚓的幼虫数和茧数均有所减少。对幼虫和茧的有效浓度（EC50值）分别为96.1和120.3 mg/kg。CTC处理显著改变了蚯蚓过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽s -转移酶（GST）的活性。丙二醛（MDA）的增加表明CTC可以引起被试蚯蚓细胞脂质过氧化。不同剂量的CTC处理后，体腔细胞单细胞凝胶电泳尾部DNA百分比作为DNA损伤的指标增加，体腔细胞DNA损伤呈剂量依赖性。综上所述，CTC可诱导蚯蚓的生理反应和遗传毒性。(C) 2012 Elsevier Ltd.版权所有。
This study aims to evaluate toxic effects of exposure to chlortetracycline (CTC) in soil on reproductive endpoints (juvenile counts and cocoon counts), biochemical responses, and genotoxic potentials of the earthworm Eisenia fetida. Results showed that juvenile counts and cocoon counts of the tested earthworms were reduced after exposure to CTC. The effective concentrations (EC50 values) for juvenile and cocoon counts were 96.1 and 120.3 mg/kg, respectively. Treatment of earthworms with CTC significantly changed the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST). An increase in malondialdehyde (MDA) indicated that CTC could cause cellular lipid peroxidation in the tested earthworms. The percentage of DNA in the tail of single-cell gel electrophoresis of coelomocytes as an indication of DNA damage increased after treatment with different doses of CTC, and a dose-dependent DNA damage of coelomocytes was found. In conclusion, CTC induces physiological responses and genotoxicity on earthworms. (C) 2012 Elsevier Ltd. All rights reserved.