The significance of apical K+ channels in mudpuppy feeding behavior.
The significance of apical K+ channels in mudpuppy feeding behavior.
复制标题
顶端钾离子通道在泥小狗进食行为中的重要性。
DOI：
10.1093/chemse/19.4.303
复制
发表时间：
1994
期刊：
影响因子：
3.5
通讯作者：
Kinnamon,SC
中科院分区：
文献类型：
--
作者：
Bowerman,AG;Kinnamon,SC
进行行为实验，以确定已知的钾通道阻滞剂，小鱼提取物（一种天然食物物质），和otber味觉刺激的mudpuppy（Necturus maculosus）的摄食行为的影响。将10-15只泥幼犬的组与溶解在明胶立方体中的以下化学品中的一种或多种一起呈现：H2O（对照）、NaCl（1.0 M; 0.2 M），粗米诺鱼提取物（0.1 g/ml），CaCl2（1.0 M），柠檬酸（0.1 M; pH 2，0.01 M; pH 3），盐酸奎宁（0.1 M），四乙基氯化铵（TEA; 0.1 M）、KCl（1.0 M）和含有L-苯丙氨酸、L-精氨酸、L-组氨酸、L-缬氨酸、浓度分别为0.02 M和0.04 M的L-谷氨酸和L-赖氨酸。将粗米诺鱼提取物离心并分离成由以下组分组成的组分：>500 D，>1000 D和>14 000 D，将每种组分呈现给立方体中的泥幼犬。结果表示为在将立方体放入口腔5分钟内排斥立方体的动物的百分比。使用Fisher精确检验的统计分析表明，含有已知阻断（CaCl 2，TEA，柠檬酸和奎宁）或渗透（KCl）mudpuppy味觉细胞中顶端K+通道的化学物质的立方体比对照立方体更厌恶，并且厌恶是浓度依赖性的。相反，含有小鱼提取物和< 14 000 D的小鱼提取物组分的立方体明显优于对照立方体。含有小鱼提取物和厌恶性化学物质的混合物的立方体比仅含有厌恶性化学物质的立方体显著更少厌恶性。这些研究结果表明，激活mudpuppy味觉细胞的顶端K+电导触发厌恶的反应，但厌恶可以改善低分子量的化合物存在于小鱼提取物。
Behavioral experiments were conducted to determine the effects of known potassium channel blockes, minnow extract (a natural food substance), and otber taste stimuli on feeding behavior in the mudpuppy (Necturus maculosus). Groups of 10–15 mudpuppies were presented with one or more of the following chemicals dissolved in gelatin cubes: H2O (control), NaCl (1.0 M; 0.2 M), crude minnow extract (0.1 g/ml), CaCl2(1.0 M), citric acid (0.1 M; pH2, 0.01 M; pH 3), quinine HCl (0.1 M), tetraemylammoninm chloride (TEA; 0.1 M), KCl (1.0 M) and an amino acid mixture containing L-phenylalanine, L-arginine, L-histidine, L-valine, L-glutamic acid and L-lysinc at concentrations of 0.02 M and 0.04 M each. Crude minnow extract was centrifuged and separated into components consisting of the following fractions: >500 D, >1000 D and >14 000 D, each of which was presented to mudpuppies in cubes. Results were expressed as the percentage of animals rejecting the cube within 5 min of taking the cube into the oral cavity. Statistical analysis using the Fisher exact test indicated that cubes containing chemicals known to block (CaCl2, TEA, citric acid and quinine) or permeate (KCl) apical K+channels in mudpuppy taste cells were more aversive than control cubes, and aversion was concentration-dependent In contrast, cubes containing minnow extract and components of minnow extract < 14 000 D were significantly preferred over control cubes. Cubes containing a mixture of minnow extract and an aversive chemical were significantly less aversive than cubes containing only an aversive chemical. These findings suggest that activation of the apical K+conductance in mudpuppy taste cells triggers an aversive response, but the aversion can be ameliorated by low molecular weight compounds present in minnow extract.