Distribution of Extracellular Flavins in a Coastal Marine Basin and Their Relationship to Redox Gradients and Microbial Community Members
Distribution of Extracellular Flavins in a Coastal Marine Basin and Their Relationship to Redox Gradients and Microbial Community Members
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DOI：
10.1021/acs.est.8b02822
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发表时间：
2018-11-06
影响因子：
11.4
通讯作者：
Sanudo-Wilhelmy, Sergio A.
中科院分区：
文献类型：
--
作者：
Monteverde, Danielle R.;Sylvan, Jason B.;Sanudo-Wilhelmy, Sergio A.
黄素（包括黄素单胞苷肽（FMN）和核黄素（RF））是一类由生物体合成的有机化合物，用于辅助关键的氧化还原反应。虽然已知在实验室培养中某些物种会分泌到细胞外，但在自然环境中黄素的浓度基本上没有报道。在这里，我们提出了孔隙水和水柱配置文件的细胞外黄素（FMN和RF）和两个降解产物（lumiflavin和lumichrome）从沿海盆地在南加州湾辅助地球化学和16 S rRNA微生物群落数据。在水柱中的皮摩尔浓度（93-300 pM FMN，14-40 pM RF）和孔隙沃茨中的低纳摩尔浓度（250-2070 pM FMN，11-210 pM RF）下可检测到黄素。高孔隙水黄素浓度随沉积物深度的增加而增加，并与总溶解铁（负）和锰（正）浓度显着相关。网络分析揭示了黄素和Dehalococcoidia和MSBL 9浮游菌的沼泽的相对丰度之间的正相关关系，表明这些谱系的成员可能分泌。这些结果表明，黄素是一个共同的组成部分，所谓的共享细胞外代谢物池，特别是在缺氧的海洋沉积物中，它们存在于生理相关的浓度为金属氧化物还原。
The flavins (including flavin mononucleotide (FMN) and riboflavin (RF)) are a class of organic compounds synthesized by organisms to assist in critical redox reactions. While known to be secreted extracellularly by some species in laboratory-based cultures, flavin concentrations are largely unreported in the natural environment. Here, we present pore water and water column profiles of extracellular flavins (FMN and RF) and two degradation products (lumiflavin and lumichrome) from a coastal marine basin in the Southern California Bight alongside ancillary geochemical and 16S rRNA microbial community data. Flavins were detectable at picomolar concentrations in the water column (93-300 pM FMN, 14-40 pM RF) and low nanomolar concentrations in pore waters (250-2070 pM FMN, 11-210 pM RF). Elevated pore water flavin concentrations displayed an increasing trend with sediment depth and were significantly correlated with the total dissolved Fe (negative) and Mn (positive) concentrations. Network analysis revealed a positive relationship between flavins and the relative abundance of Dehalococcoidia and the MSBL9 Glade of Planctomycetes, indicating possible secretion by members of these lineages. These results suggest that flavins are a common component of the so-called shared extracellular metabolite pool, especially in anoxic marine sediments where they exist at physiologically relevant concentrations for metal oxide reduction.