Pharmacognostical study of Desmodium caudatum.
Pharmacognostical study of Desmodium caudatum.
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DOI：
10.1590/0001-3765201920180637
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发表时间：
2019-06
影响因子：
1.3
通讯作者：
Junni Li;Xiao-jing Lin;Gengqiu Tang;Rongsheng Li;Dong Wang;Shengguo Ji
中科院分区：
文献类型：
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作者：
Junni Li;Xiao-jing Lin;Gengqiu Tang;Rongsheng Li;Dong Wang;Shengguo Ji
尾草（Desmodium caudatum (Thunb.) DC）是一种常绿植物，在我国中部和南部地区广泛使用，具有很高的经济价值，具有治疗发烧、痢疾、胃肠炎、脱肛、蛇咬伤、乳腺炎、疖痈等功效。尽管其作为中药用途广泛，但目前尚未见关于尾金钱草鉴定的系统研究报道。本研究采用药材来源及形态性状、药材性状、显微性状、理化参数测定及植物化学筛选、DNA条形码分析等附加药学鉴定方法，建立了准确、有效的药材鉴别体系。此外，为了更准确地鉴定样品，还采用了分子生药学研究。选择并评估了核基因组的 ITS 基因座和叶绿体基因组的 psbA-trnH 基因座，它们是变异最大的基因座。该研究将有利于质量标准的制定和物种的鉴定。
Desmodium caudatum (Thunb.) DC, is an ever-green plant widely used in the central and southern China with great economic value for their medical values on fever, dysentery, gastroenteritis, rectal prolapse, snake bites, mastitis, and boils carbuncle. Despite its extensive uses as a traditional Chinese medicine, no systematic research on the identification of Desmodium caudatum has been reported. In this study, traditional pharmacognostical identification including the botanical origin and morphological characters, medicinal material characters, microscopic characters, physicochemical parameters determination and phytochemical screening, and DNA barcoding analysis were employed to establish an accurate and effective identification system of Desmodium caudatum. In addition, the molecular pharmacognosy study was adopted in order to identify the samples more accurately. The ITS loci of the nuclear genome and psbA-trnH loci of the chloroplast genome were selected and evaluated, which were the most variable loci. The study will be beneficial to the development of the quality standard and the identification of species.