Development of hamster tracheal epithelium: IV. Cell proliferation and cytodifferentiation in the neonate.
Development of hamster tracheal epithelium: IV. Cell proliferation and cytodifferentiation in the neonate.
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仓鼠气管上皮的发育：IV。
DOI：
10.1002/ar.1092140213
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
McDowell,EM
中科院分区：
文献类型：
--
作者：
Otani,EM;Newkirk,C;McDowell,EM
研究了新生仓鼠从出生当天（第1天）至第8天气管上皮的发育情况。形态学变化和细胞（基底、分泌、纤毛）的比例以及沿背侧和腹侧上皮表面的有丝分裂指数被量化。细胞比例在一周内保持稳定，但背侧和腹侧上皮的组成不同。腹侧上皮由约59%的分泌细胞、39%的基底细胞和2%的纤毛细胞组成，而背侧上皮由约52%的分泌细胞、32%的基底细胞和16%的纤毛细胞组成。有丝分裂指数普遍小于1%，分泌细胞的有丝分裂活性与背、腹侧上皮分泌细胞和基底细胞的比例成正比。在产后第一周发生的变化与基底细胞和分泌细胞的成熟有关。在一周的前半段，糖原从两种细胞中迅速丢失，侧细胞膜变得越来越复杂。分泌细胞的顶端微绒毛在第2天形成，基底细胞的半丝粒发育良好。分泌细胞内粗糙光滑的内质网膜迅速发育，部分细胞在第4-8天出现大量黏液颗粒，尤其是软骨环之间的腹侧上皮。研究表明，在出生后的第一周内，气管背侧或腹侧上皮的基底细胞、分泌细胞和纤毛细胞的比例不会发生变化，但此时基底细胞和分泌细胞经历了快速的细胞分化和功能成熟。
The development of the tracheal epithelium was studied in neonatal hamsters beginning on the day of birth (day 1) and ending on day 8. Morphological changes were characterized and the proportions of cells (basal, secretory, ciliated) and mitotic indices were quantified along dorsal and ventral epithelial surfaces. Cellular proportions were stable throughout the week but the makeup of the dorsal and ventral epithelia was different. The ventral epithelium was composed of about 59% secretory cells, 39% basal cells, and 2% ciliated cells, whereas the dorsal epithelium was composed of about 52% secretory cells, 32% basal cells, and 16% ciliated cells. Mitotic indices were generally less than 1% and mitotic activity in secretory cells predominated proportionate to the ratios of secretory cells and basal cells in dorsal and ventral epithelia. During the first postnatal week changes occurred that were related to the maturation of basal cells and secretory cells. Glycogen was rapidly lost from both cell types during the first part of the week and the lateral cell membranes became increasingly complex. Apical microvilli had formed in the secretory cells by day 2 and hemidesmosomes were well developed in the basal cells. Rough and smooth endoplasmic reticulum membranes developed rapidly in the secretory cells, and mucous granules were abundant in some cells on days 4—8, especially in the ventral epithelium between the cartilage rings.The study shows that shifts in the proportions of basal, secretory, and ciliated cells do not occur in dorsal or ventral tracheal epithelium during the first postnatal week but the basal cells and secretory cells undergo rapid cytodifferentiation and functional maturation at this time.