Diamagnetic levitation changes growth, cell cycle, and gene expression of Saccharomyces cerevisiae
Diamagnetic levitation changes growth, cell cycle, and gene expression of Saccharomyces cerevisiae
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DOI：
10.1002/bit.21526
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发表时间：
2007-11-01
影响因子：
3.8
通讯作者：
Hammond, Timothy G.
中科院分区：
文献类型：
--
作者：
Coleman, Chasity B.;Gonzalez-Villalobos, Romer A.;Hammond, Timothy G.
非均匀磁场被用于磁阱中，通过利用几乎所有材料的天然抗磁性来悬浮生物标本。使用酿酒酵母，本报告调查是否磁场（B）诱导生长，细胞周期和基因表达的变化。比较重力和温度的影响，可以确定反应是一般途径还是刺激特异性的。在野生型（WT）酵母和转录因子Msn4或Sfp1缺失的菌株中检查生长和细胞周期分析。Msn4、Sfp1和Rap1与对物理力的反应有关，但只有Msn4和Sfp1缺失是可行的。在听到GFP标记的YIL052C（Sfp1依赖性）、YST-2（Sfp1/Rap1依赖性）或SSA4（Msn4依赖性）报告基因的菌株中检查基因表达变化。细胞生长和基因表达反应是高度刺激特异性的。B仅在Msn4或Sfp 1缺失后增加生长，与细胞周期的G1和G2/M降低和S期增加相关。此外，B抑制YIL 052 C和YST 2的表达。重力下降的Sfp1，但不是Msn4依赖的方式，在与减少G2/M和增加S期的细胞周期的增长。此外，重力降低了SSA4和YIL052C基因的表达。温度以Msn4-和Sfp1-依赖的方式增加细胞生长，并增加G1和G2/M，减少细胞周期的S期。此外，温度增加YIL052C基因的表达。这项研究表明，B对细胞生长、细胞周期和基因表达具有选择性作用，这些作用是刺激特异性的。
Inhomogeneous magnetic fields are used in magnetic traps to levitate biological specimens by exploiting the natural diamagnetism of virtually all materials. Using Saccharomyces cerevisiae, this report investigates whether magnetic field (B) induces changes in growth, cell cycle, and gene expression. Comparison to the effects of gravity and temperature allowed determination of whether the responses are general pathways or stimulus specific. Growth and cell cycle analysis were examined in wild-type (WT) yeast and strains with deletions in transcription factors Msn4 or Sfp1. Msn4, Sfp1, and Rap1 have been implicated in responses to physical forces, but only Msn4 and Sfp1 deletions are viable. Gene expression changes were examined in strains hearing GFP-tagged reporters for YIL052C (Sfp1-dependent), YST-2 (Sfp1/Rap1-dependent), or SSA4 (Msn4-dependent). The cell growth and gene expression responses were highly stimulus specific. B increased growth only following Msn4 or Sfp1 deletion, associated with decreased G1 and G2/M and increased S phase of the cell cycle. In addition, B suppressed expression of both YIL052C and YST2. Gravity decreased growth in an Sfp1 but not Msn4-dependent manner, in association with decreased G2/M and increased S phase of the cell cycle. Additionally, gravity decreased expression of SSA4 and YIL052C genes. Temperature increased cell growth in an Msn4- and Sfp1-dependent manner in association with increased G1 and G2/M with decreased S phase of the cell cycle. In addition, temperature increased YIL052C gene expression. This study shows that B has selective effects on cell growth, cell cycle, and gene expression that are stimulus specific.