Refinement of pepsin digestion method for isolation of Toxoplasma gondii from infected tissues
Refinement of pepsin digestion method for isolation of Toxoplasma gondii from infected tissues
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DOI：
10.1016/s0304-4017(97)00135-0
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发表时间：
1998-01-15
影响因子：
2.6
通讯作者：
Dubey, JP
中科院分区：
文献类型：
--
作者：
Dubey, JP
对酸性胃酶消化法进行了改进，以从动物肌肉组织中分离弓形虫。肌肉孵育60min而不是90min，用1.2%(w/v)的碳酸氢钠中和酸液，反复洗涤和离心法。爱思唯尔科学公司出版。
Modification of the acid pepsin digestion procedure to isolate Toxoplasma gondii from animal muscular tissues is described. Muscles were incubated for 60 min instead of 90 min and acid was neutralized with 1.2% (w/v) sodium bicarbonate instead by repeated washings and centrifugations. Published by Elsevier Science B.V.