Coactivation of secretogranin-II and BDNF genes mediated by calcium signals in mouse cerebellar granule cells
Coactivation of secretogranin-II and BDNF genes mediated by calcium signals in mouse cerebellar granule cells
复制标题
DOI：
10.1016/s0169-328x(98)00299-x
复制
发表时间：
1999-01-08
期刊：
影响因子：
--
通讯作者：
Tsuda, M
中科院分区：
文献类型：
--
作者：
Fujita, Y;Katagi, J;Tsuda, M
在小鼠小脑颗粒细胞的原代培养中，脑源性神经营养因子（BDNF）基因以活性依赖性方式被激活，伴随着Ca2+通过电压依赖性钙通道（VDCC）流入神经元。在本研究中，我们通过与 BDNF 和 c-fos 基因进行比较，研究了分泌颗粒素-II (Sg-II) 基因在通过 VDCC 诱发的 Ca2+ 信号方面的诱导能力。分别通过降低和重新升高细胞外氯化钾 (KCI) 浓度来剥夺和随后诱导膜去极化，导致 Sg-II、BDNF 和 c-fos mRNA 表达先降低后升高。 Sg-II mRNA 表达的增加早于 BDNF 检测到，但较慢。 Sg-II mRNA 表达的增加取决于细胞外 Ca2+ 的诱导，并受到尼卡地平的抑制，表明 Sg-II 和 BDNF 基因的诱导需要 Ca2+ 通过 VDCC 流入。放线菌酮对蛋白质从头合成的抑制并不影响 Sg-II 的诱导。 Sg-II基因对细胞外KCl浓度变化的响应与BDNF相同，但与c-fos基因不同。因此，Sg-II 基因与 BDNF 基因共同激活，以响应通过 VDCC 的 Ca2+ 流入引起的细胞内 Ca2+ 信号。 (C) 1999 Elsevier Science B.V. 保留所有权利。
In primary culture of mouse cerebellar granule cells, the brain-derived neurotrophic factor (BDNF) gene is activated in an activity-dependent manner, accompanying Ca2+ influx into neurons through voltage-dependent calcium channels (VDCCs). In this study, we investigated the inducibility of secretogranin-II (Sg-II) gene in terms of Ca2+ signals evoked via VDCCs, by a comparison with BDNF and c-fos genes. Deprivation and subsequent induction of membrane depolarization by lowering and reelevating the extracellular concentration of potassium chloride (KCI), respectively, led to an decrease and then an increase in the Sg-II, BDNF and c-fos mRNA expression. The increase in Sg-II mRNA expression was detected as early as but was slower than that of BDNF one. The increase in Sg-II mRNA expression was induced depending upon the extracellular Ca2+ and inhibited by nicardipine, indicating a requirement of Ca2+ influx through VDCCs for the Sg-II as well as BDNF gene induction. Inhibition of de novo protein synthesis by cycloheximide did not affect the Sg-II induction. The response of Sg-II gene to the changes in extracellular KCl concentration was the same as that of BDNF but different from that of c-fos gene. Thus, Sg-II gene is coactivated with BDNF gene in response to the intracellular Ca2+ signals evoked via Ca2+ influx through VDCCs. (C) 1999 Elsevier Science B.V. All rights reserved.