Identification and characterization of long non-coding RNA in prenatal and postnatal skeletal muscle of sheep
Identification and characterization of long non-coding RNA in prenatal and postnatal skeletal muscle of sheep
复制标题
绵羊产前和产后骨骼肌中长链非编码RNA的鉴定和表征
DOI：
10.1016/j.ygeno.2018.01.009
复制
发表时间：
2019-03-01
期刊：
影响因子：
4.4
通讯作者：
Hu, Shengwei
中科院分区：
文献类型：
--
作者：
Li, Cun-Yuan;Li, Xiaoyue;Hu, Shengwei
lncRNAs are a class of transcriptional RNA molecules of > 200 nucleotides in length. However, the overall expression pattern and function of lncRNAs in sheep muscle is not clear. Here, we identified 1566 lncRNAs and 404 differentially expressed lncRNAs in sheep muscle from prenatal (110 days of fetus) and postnatal (2 to 3 years old of adult sheep) developmental stages by using RNA-seq technology. Several lncRNAs were identified by using RT-PCR and DNA sequencing. The expression levels of several lncRNAs were confirmed by qRT-PCR. We analyzed the effect of lncRNAs that act cis to the target genes. lncRNA targeting genes were involved in signaling pathways associated with growth and development of muscle by GO and KEGG enrichment analysis. Through our study, we provide a comprehensive expression profile of muscle lncRNAs in sheep, which provides valuable resources for further understanding genetic regulation of muscle growth and development from the perspective of lncRNA.
