Resistance to dieldrin plus fipronil assorts with chromosome inversion 2La in the malaria vector Anopheles gambiae
Resistance to dieldrin plus fipronil assorts with chromosome inversion 2La in the malaria vector Anopheles gambiae
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DOI：
10.1046/j.1365-2915.2000.00222.x
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发表时间：
2000-06-01
影响因子：
1.9
通讯作者：
Coetzee, M
中科院分区：
文献类型：
--
作者：
Brooke, BD;Hunt, RH;Coetzee, M
非洲一些地区曾报告冈比亚按蚊复合体(双翅目：蚊科)疟疾媒介蚊子对环二烯类杀虫剂的抗药性。我们报告了两个实验室菌株对环二烯狄氏剂的耐药性。冈比亚原产冈比亚，代号为Ian P20，来自尼日利亚(1979)，代号CIG，来自科特迪瓦(1997)。在成年雌性蚊虫中，狄氏剂抗性水平很高(40-75%的雌蚊在4%狄氏剂中1小时存活)，并与染色体旁着丝粒倒位2LA密切相关。这种倒置没有发生在Hardy-Weinberg比例中，但在两个品系中都显示出过多的杂合子，这可能是造成高水平抗性的原因。这一连锁也表明Dieldrin抗性在Ian P20中占主导地位。伊恩P20菌株的亚种样品分别用4%狄氏剂或2%氟虫腈处理1h后，死亡率(61%和65%)无显著差异。大多数接受氟虫腈治疗的幸存者在随后的狄氏剂暴露中也存活了下来(46/50=92%)。这两类杀虫剂(环二烯和苯基吡唑)之间的这种明显的交叉抗药性对控制蚊虫野生种群的抗药性具有重要意义。冈比亚复合体。
Cyclodiene insecticide resistance in malaria vector mosquitoes of the Anopheles gambiae species complex (Diptera: Culicidae) has been reported previously from several parts of Africa. We report resistance to dieldrin, a cyclodiene, in two laboratory strains of An. gambiae Giles sensu stricto code-named Ian P20 from Nigeria (1979) and CIG from Cote d'Ivoire (1997). Dieldrin resistance levels were high in adult female mosquitoes (40-75% survived exposure to 4% dieldrin for 1 h) and was closely linked with chromosomal paracentric inversion 2La. This inversion did not occur in Hardy-Weinberg proportions, but showed an excess of heterozygotes in both strains, which may account for the high levels of resistance. This linkage also suggests that dieldrin resistance in Ian P20 is dominant. After subsamples of strain Ian P20 were exposed for 1 h to dieldrin 4% or fipronil 2% (discriminating concentrations), the resultant mortality-rates (61% and 65%) were not significantly different. Most survivors after fipronil treatment also survived subsequent exposure to dieldrin (46/50 = 92%). This apparent cross-resistance between insecticides of two classes (cyclodiene and phenyl pyrazole) has implications for the management of insecticide resistance in wild populations of the An. gambiae complex.