Changes in CD4+, CD8+, CD4+CD8+, and immunoglobulin M-positive peripheral blood mononuclear cells of postweaning multisystemic wasting syndrome-affected pigs and age-matched uninfected wasted and healthy pigs correlate with lesions and porcine circovirus type 2 load in lymphoid tissues
Changes in CD4+, CD8+, CD4+CD8+, and immunoglobulin M-positive peripheral blood mononuclear cells of postweaning multisystemic wasting syndrome-affected pigs and age-matched uninfected wasted and healthy pigs correlate with lesions and porcine circovirus type 2 load in lymphoid tissues
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DOI：
10.1128/cdli.9.2.236-242.2002
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发表时间：
2002-03-01
期刊：
影响因子：
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通讯作者：
Mateu, E
中科院分区：
文献类型：
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作者：
Darwich, L;Segalés, J;Mateu, E
从几个有断奶后多系统衰竭综合征（PMWS）病史的常规猪场中选择41头8 ~ 12周龄的废猪，根据原位杂交（ISH）检测结果，将其分为两组。24头猪检测为PCV 2阳性（PCV 12阳性组），而17头猪检测为PCV 2阴性（PCV 2阴性组）。另外，8头来自实验农场的未感染的健康猪被用作对照。采集肝素化血液样本以获得外周血单核细胞。使用适当的单克隆抗体，通过流式细胞术分析CD 4（+）、CD 8（+）、CD 4（+）CD 8（+）（双阳性[DP]）和免疫球蛋白M阳性（IgM（+））细胞亚群。进行了组织学研究，以评价不同淋巴器官（腹股沟浅表淋巴结和肠系膜淋巴结、派伊尔集合淋巴结、扁桃体和脾脏）中淋巴细胞耗竭的表观程度，并使用ISH技术测定PCV 2基因组的病毒载量。PCV 2阳性组动物的CD 8（+）和DP细胞亚群与其他组相比显著下降（P < 0.05）。PCV 2阳性猪淋巴组织中PCV 2基因组的数量与淋巴组织中细胞耗竭的程度以及外周血中IgM（+）和CD 8（+）细胞的相对减少有关（P < 0.05）。这些数据支持PCV 2阳性猪可能具有受损的免疫应答的观点。
Forty-one 8- to 12-week-old wasted pigs were selected from several conventional farms with histories of postweaning multisystemic wasting syndrome (PMWS) and classified into two groups according to their porcine circovirus type 2 (PCV2) infection status, as determined by in situ hybridization (ISH). Twenty-four pigs tested positive for PCV2 (PCV12-positive group), while 17 pigs tested negative for PCV2 (PCV2-negative group). In addition, eight uninfected healthy pigs from an experimental farm were used as controls. Heparinized blood samples were taken to obtain peripheral blood mononuclear cells. The CD4(+), CD8(+), CD4(+) CD8(+) (double-positive [DP]), and immunoglobulin M-positive (IgM(+)) cell subsets were analyzed by flow cytometry with appropriate monoclonal antibodies. Histopathological studies were done to evaluate the apparent degrees of lymphocyte depletion in different lymphoid organs (superficial inguinal and mesenteric lymph nodes, Peyer's patches, tonsils, and spleen) and to determine the viral load of the PCV2 genome by using an ISH technique. Animals of the PCV2-positive group showed a significant downshift of the CD8(+) and DP cell subsets compared to the other groups (P < 0.05). Moreover, in PCV2-positive pigs, the amount of PCV2 genome in lymphoid tissues was related to the degree of cell depletion in those tissues (P < 0.05) as well as to the relative decrease in IgM(+) and CD8(+) cells in peripheral blood. These data support the notion that PCV2-positive pigs might have an impaired immune response.