Investigation into the effect that probe immobilisation method type has on the analytical signal of an EIS DNA biosensor.
Investigation into the effect that probe immobilisation method type has on the analytical signal of an EIS DNA biosensor.
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DOI：
10.1016/j.bios.2006.05.021
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发表时间：
2007-02
影响因子：
12.6
通讯作者：
B. Lillis;M. Manning;Eileen Hurley;H. Berney;R. Duane;A. Mathewson;M. Sheehan
中科院分区：
文献类型：
--
作者：
B. Lillis;M. Manning;Eileen Hurley;H. Berney;R. Duane;A. Mathewson;M. Sheehan
研究了用于DNA传感器开发的增强表面积电解质绝缘体半导体（EIS）器件的分析性能。通过监测靶杂交检测过程中DNA探针层对阻抗信号的影响，提高了EIS的性能。两个普遍采用的共价化学，直接和间隔介导的连接氨基修饰的探针分子的氨基功能化的表面进行了研究。使用落射荧光显微镜在平面和增强表面积基底上测量固定化探针的相对面密度。观察到每种固定方法的再现性对阻抗信号的再现性有直接影响。的灵敏度和选择性被认为是依赖于固定方法的类型。真实的实时阻抗检测靶DNA杂交浓度低至25和1 nM是可能的。还研究了探针浓度对选择性和非选择性相互作用的阻抗信号的影响。
The analytical performance of an enhanced surface area electrolyte insulator semiconductor (EIS) device was investigated for DNA sensor development. The work endeavored to advance EIS performance by monitoring the effect of DNA probe layers have on the impedimetric signal during target hybridisation detection. Two universally employed covalent chemistries, direct and spacer-mediated attachment of amino modified probe molecules to amino-functionalised surfaces were investigated. Relative areal densities of immobilised probe were measured on planar and enhanced surface area substrates using epi-fluorescence microscopy. The reproducibility of the each immobilisation method was seen to have a direct effect on the reproducibility of the impedimetric signal. The sensitivity and selectivity was seen to be dependent on the type of immobilisation method. Real time, impedimetric detection of target DNA hybridisation concentrations as low as 25 and 1nM were possible. The impact that probe concentration had on the impedimetric signal for selective and non-selective interactions was also investigated.