Effect of nourishing "Yin"-removing "Fire" Chinese herbal mixture on hypothalamic kisspeptin expression in female precocious rats
Effect of nourishing "Yin"-removing "Fire" Chinese herbal mixture on hypothalamic kisspeptin expression in female precocious rats
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DOI：
10.1016/j.jep.2009.11.009
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发表时间：
2010-02-03
影响因子：
5.4
通讯作者：
Tian, Zhanzhuang
中科院分区：
文献类型：
--
作者：
Sun, Yan;Perry, Genevieve Neal;Tian, Zhanzhuang
目的：观察滋阴祛火中药合剂对达那唑诱导的雌性性性早熟大鼠下丘脑Kispeptin表达的影响。材料与方法：雌性SD大鼠分为正常对照组(N)、中枢性早熟模型(M)、无性早熟模型组(V)、中枢性早熟模型组(HM)和生理盐水组(S)。出生后第5天，单次皮下注射达那唑300 mg建立慢性阻塞性肺疾病模型。从P15开始，HM组大鼠连续灌胃1ml/50g体重混合物，直到建立连续的两个有规律的发情周期。结果：与M组和S组相比，HM组大鼠阴道开放时间和两个发情周期的建立时间明显延迟(P均<0.05)。在发病-青春期，HM组下丘脑Kiss-1mRNA水平及弓状核(ARC)、视前区(POA)和脑室周围核(PEN)Kispeptin免疫反应阳性细胞数明显低于M组和S组(P均<0.01)。结论：Kispeptin信号转导通路可能参与了中药合剂治疗CPP的作用。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
Aim: The present study aims to investigate the effect of nourishing "Yin"-removing "Fire" herbal mixture, a Chinese herb-based formulation, on hypothalamic kisspeptin expression in danazol-induced female precocious model rats.Materials and methods: The female Sprague-Dawley rats were divided into intact normal (N), central precocious puberty (CPP) model (M), vehicle without CPP (V), CPP model exposed to herbal mixture (HM) and CPP model exposed to saline (S) groups. At postnatal day 5, a single subcutaneous injection of 300 mu g of danazol was administered to induce CPP model rats. From P15, rats in the HM group were continuously gavaged with the 1 ml/50 g body weight mixture, until two consecutive regular estrous cycles were established. The hypothalamic Kiss-1 expression was detected by RT-PCR and immunohistochemistry.Results: The day of vaginal opening and establishment of two regular estrous cycles were delayed in the HM group compared with M and S groups (P < 0.05, respectively). The level of hypothalamic Kiss-1 mRNA and the number of kisspeptin-immunoreactive (kisspeptin-ir) cells in the arcuate nucleus (ARC), preoptic area (POA) and periventricular nucleus (PeN), were decreased significantly in the HM group compared with the M and S groups (P < 0.01, respectively) on the day of onset-puberty.Conclusion: These results indicate that the kisspeptin signaling pathway might be involved in the effect of herbal mixture treatment on CPP. (C) 2009 Elsevier Ireland Ltd. All rights reserved.