Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step
Enhancement of cellulose saccharification kinetics using an ionic liquid pretreatment step
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DOI：
10.1002/bit.21047
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发表时间：
2006-12-05
影响因子：
3.8
通讯作者：
Schall, Constance A.
中科院分区：
文献类型：
--
作者：
Dadi, Anantharam P.;Varanasi, Sasidhar;Schall, Constance A.
在水介质中由纤维素酶系统催化的纤维素水解成葡萄糖的反应速率缓慢，这在很大程度上是由于纤维素的高度结晶结构和酶吸附位点的不可接近性。在这项研究中，尝试使用离子液体（IL）、氯化1-正丁基-3-甲基咪唑鎓来破坏纤维素结构，以加速随后的水解反应的纤维素再生策略。离子液体是一类新型非挥发性溶剂，具有独特的溶剂化特性。它们可以调节以溶解包括纤维素在内的多种化合物。由于挥发性极低，与大多数其他挥发性溶剂系统相比，IL 预计对空气质量的环境影响最小。通过可溶性还原糖测定测量，与未处理的纤维素 (Avicel PH-101) 相比，再生纤维素的初始酶水解速率大约高 50 倍。 (c) 2006 年 Wiley 期刊公司
Hydrolysis of cellulose to glucose in aqueous media catalyzed by the cellulase enzyme system suffers from slow reaction rates due in large part to the highly crystalline structure of cellulose and inaccessibility of enzyme adsorption sites. In this study, an attempt was made to disrupt the cellulose structure using the ionic liquid (IL), 1-n-butyl-3-methylimidazolium chloride, in a cellulose regeneration strategy which accelerated the subsequent hydrolysis reaction. ILs are a new class of non-volatile solvents that exhibit unique solvating properties. They can be tuned to dissolve a wide variety of compounds including cellulose. Because of their extremely low volatility, ILs are expected to have minimal environmental impact on air quality compared to most other volatile solvent systems. The initial enzymatic hydrolysis rates were approximately 50-fold higher for regenerated cellulose as compared to untreated cellulose (Avicel PH-101) as measured by a soluble reducing sugar assay. (c) 2006 Wiley Periodicals, Inc.