Molecular identification of Bacteria and Eukarya inhabiting an Antarctic cryoconite hole
Molecular identification of Bacteria and Eukarya inhabiting an Antarctic cryoconite hole
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DOI：
10.1007/s00792-002-0309-0
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发表时间：
2003-06-01
期刊：
影响因子：
2.9
通讯作者：
Reeve, JN
中科院分区：
文献类型：
--
作者：
Christner, BC;Kvitko, BH;Reeve, JN
Inhabitants of a cryoconite hole formed in the Canada Glacier in the McMurdo Dry Valley region of Antarctica have been isolated and identified by small subunit (16S/18S) rDNA amplification, cloning, and sequencing. The sequences obtained revealed the presence of members of eight bacterial lineages (Acidobacterium, Actinobacteria, Cyanobacteria, Cytophagales, Gemmimonas, Planctomycetes, Proteobacteria, and Verrucomicrobia) and metazoan (nematode, tardigrade, and rotifer), truffle (Choiromyces), ciliate (Spathidium), and green algal (Pleurastrium) Eukarya. Bacterial recovery was similar to20-fold higher at 4 degreesC and 15 degreesC than at 22 degreesC, and obligately psychrophilic bacteria were identified and isolated. Several of the rDNA molecules amplified from isolates and directly from cryoconite DNA preparations had sequences similar to rDNA molecules of species present in adjacent lake ice and microbial mat environments. This cryoconite hole community was therefore most likely seeded by particulates from these local environments. Cryoconite holes may serve as biological refuges that, on glacial melting, can repopulate the local environments.
