Cap1p is involved in multiple pathways of oxidative stress response in Candida albicans
Cap1p is involved in multiple pathways of oxidative stress response in Candida albicans
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Cap1p 参与白色念珠菌氧化应激反应的多种途径
DOI：
10.1016/j.freeradbiomed.2005.11.019
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发表时间：
2006-04-01
影响因子：
7.4
通讯作者：
Jiang, YY
中科院分区：
文献类型：
--
作者：
Wang, Y;Cao, YY;Jiang, YY
Cap 1 p是白念珠菌的一种转录因子，被认为参与氧化应激耐受，但涉及的途径仍不清楚。该研究旨在通过使用微阵列分析检测H2 O2处理后cap 1缺失菌株CJD 21及其亲本菌株CAI 4的转录谱变化来揭示可能的途径。在鉴定的89个基因中，有76个基因在暴露于H2 O2后在CAI 4中差异表达，在Cap I p依赖性表达模式中。我们已经证明Cap I p参与了C.白念珠菌通过多种途径，包括细胞抗氧化防御系统（例如，硫氧还蛋白还原酶，谷胱甘肽还原酶，谷胱甘肽S-转移酶），碳水化合物代谢和能量代谢（例如，葡萄糖-6-磷酸脱氢酶、转醛酶、谷胱甘肽酶1、NADH依赖性黄素氧化还原酶），蛋白质降解（例如，26 S蛋白酶体调节亚基，泛素特异性蛋白酶），ATP依赖性RNA解旋酶（例如，DEAD盒蛋白ATP依赖性RNA解旋酶）和抗性途径（例如，多药耐药蛋白，镉抗性所必需的ABC转运蛋白）。实时荧光定量RT-PCR分析进一步证实了芯片的结果。总的来说，这项研究提供了新的见解帽IP在氧化应激反应中的生物学功能。(c)2005年爱思唯尔公司All rights reserved.
Cap1p, a transcription factor in Candida albicans, is thought to participate in oxidative stress tolerance, but the pathways involved are still unclear. The Study was designed to reveal the possible pathways by examining changes in the transcription profile after H2O2 treatment with both the cap1-deleted strain CJD21 and its parental strain CAI4 using microarray analysis. Of the identified 89 genes differentially expressed in CAI4 after exposure to H2O2, 76 genes were in a Cap I p-dependent expression pattern. We have shown that Cap I p is involved in the oxidative stress response in C. albicans via multiple pathways, including the cellular antioxidant defense system (e.g., thioredoxin reductase, glutathione reductase, glutathione S-transferase), carbohydrate metabolism and energy metabolism (e.g., glucose-6-phosphate dehydrogenase, transaldolase, glyoxalase 1, NADH-dependent flavin oxidoreductase), protein degradation (e.g., 26S proteasome regulatory subunit, ubiquitin-specific protease), ATP-dependent RNA helicase (e.g., DEAD box protein ATP-dependent RNA helicase), and resistance pathways (e.g., multidrug resistance protein, ABC transporter essential for cadmium resistance). Real-time reverse transcription-PCR analysis further confirmed the results of microarray. Collectively, this study provides new insight into the biological functions of Cap I p in oxidative stress response. (c) 2005 Elsevier Inc. All rights reserved.