Reply to Chien: Clarification of the effect of ligand on γδ-TCR repertoire selection.
Reply to Chien: Clarification of the effect of ligand on γδ-TCR repertoire selection.
复制标题
回复Chien：澄清配体对γδ-TCR 库选择的影响。
DOI：
10.1073/pnas.1804193115
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发表时间：
2018
影响因子：
11.1
通讯作者：
Wiest,DavidL
中科院分区：
文献类型：
--
作者：
Fahl,ShawnP;Wiest,DavidL
在我们最近的出版物(1)中，我们报告了先前发现促进T22反应的EGYEL基序从H-2T缺陷小鼠发育的成熟的γδ-T细胞池中被耗尽，表明配体正在影响γδ-T细胞受体(TCR)的谱系(1)。在她的信中，Chien(2)建议这一结论是无效的，因为在报告我们的分析时，我们没有将上游色氨酸(W)残基包括在基序中，她认为这对T22-反应性(2)至关重要。我们重新分析了数据，数据显示W…EGYEL基序及其所有排列都从H-2T缺陷小鼠中发育的CD24低种群中消失(图1)。正如预期的那样，这个基序在四聚体−胸腺γδ细胞中的背景水平上被发现。关于W残基对T22-反应活性至关重要的观点，虽然先前报道色氨酸到丙氨酸的取代在表面等离子共振分析中确实大大减少了与T22的结合，但这并不排除该位置上其他氨基酸的相容性(3)。
In our recent publication (1), we report that the EGYEL motif, previously found to promote T22-reactivity, was depleted from the mature CD24 low pool of γδ-T cells that developed in H-2T–deficient mice, indicating that ligand was influencing the γδ-T cell receptor (TCR) repertoire (1). In her letter, Chien (2) suggests that this conclusion was invalid because, in reporting our analysis, we did not include the upstream tryptophan (W) residue in the motif, which she contends is critical for T22-reactivity (2). We have reanalyzed the data, which reveal that the W… EGYEL motif and all permutations thereof, are depleted from the CD24 low population that developed in H-2T–deficient mice (Fig. 1). This motif is found at background levels in tetramer− thymic γδ cells, as expected. Regarding the contention that the W residue is essential to T22-reactivity, while it was previously reported that a tryptophan to alanine replacement did substantially reduce binding to T22 in surface plasmon resonance analysis, this does not exclude the compatibility of other amino acids at this position (3).