Boosting of activity enhancement of K+-responsive quadruplex hammerhead ribozyme
Boosting of activity enhancement of K+-responsive quadruplex hammerhead ribozyme
复制标题
K反应性四链锤头核酶活性增强的促进
DOI：
10.1039/c5cc00961h
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发表时间：
2015
影响因子：
4.9
通讯作者：
Takashi Nagata and Masato Katahira
中科院分区：
文献类型：
--
作者：
Yudai Yamaoki;Tsukasa Mashima;Takashi Nagata and Masato Katahira
开发了两种第二代四链体锤头状核酶，其活性通过嵌入的r（GGA）3 GG的四链体形成而响应K+而增强。当K+不存在时，应用不同的策略来抑制基础活性。结果表明，K+的加入使酶的活性提高了21倍。
Two second-generation quadruplex hammerhead ribozymes, whose activity enhances in response to K+via quadruplex formation of embedded r(GGA)3GG, were developed. Different strategies were applied to suppress basal activity when K+ is absent. As a result, the activity enhancement upon the addition of K+ has reached as high as 21-fold.