In vitro evaluation of antibiotic synergy for NDM-1-producing Enterobacteriaceae
In vitro evaluation of antibiotic synergy for NDM-1-producing Enterobacteriaceae
复制标题
DOI：
10.1093/jac/dkr296
复制
发表时间：
2011-10-01
影响因子：
5.2
通讯作者：
Nordmann, Patrice
中科院分区：
文献类型：
--
作者：
Bercot, Beatrice;Poirel, Laurent;Nordmann, Patrice
目的：分析粘菌素、磷霉素和替加环素单独或联合使用对产NDM-1肠杆菌的体外活性。方法：测定粘菌素、磷霉素和替加环素对28株产NDM-1肠杆菌的MIC值。采用微量稀释和棋盘技术对 8 种临床分离株和 1 种大肠杆菌转接合子（其中 6 种对三种抗生素敏感）进行了体外协同组合测试。 结果：测定了粘菌素、磷霉素和替加环素的 MIC，显示三分之一的产生 NDM-1 的分离株对至少一种耐药或中间耐药。 三种药物。然而，在极少数情况下观察到粘菌素加磷霉素和粘菌素加替加环素的体外协同活性。结论：在极少数情况下观察到粘菌素和磷霉素以及粘菌素和替加环素的协同活性，大多数相互作用是无关紧要的。
Objectives: To analyse the in vitro activity of colistin, fosfomycin and tigecycline alone or in combination against enterobacterial NDM-1 producers.Methods: MIC values of colistin, fosfomycin and tigecycline were determined for 28 NDM-1-producing enterobacterial isolates. In vitro synergy combination testing was performed for eight clinical isolates and one Escherichia coli transconjugant (six being susceptible to the three antibiotics) using microdilution and chequerboard techniques.Results: MICs of colistin, fosfomycin and tigecycline were determined, showing that one-third of NDM-1-producing isolates were resistant or intermediate to at least one of the three drugs. Nevertheless, in vitro synergistic activity was observed for colistin plus fosfomycin and colistin plus tigecycline in very rare cases.Conclusions: Synergistic activity was observed for colistin and fosfomycin, and colistin and tigecycline in rare cases, most of the interactions being indifferent.