A CDNA CLONE FOR A NOVEL NUCLEAR-PROTEIN WITH DNA-BINDING ACTIVITY
A CDNA CLONE FOR A NOVEL NUCLEAR-PROTEIN WITH DNA-BINDING ACTIVITY
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DOI：
10.1007/bf00360539
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发表时间：
1992-10-01
期刊：
影响因子：
1.6
通讯作者：
LEE, JS
中科院分区：
文献类型：
--
作者：
MATTIONI, T;HUME, CR;LEE, JS
为了鉴定调节特定基因的反式作用因子，我们克隆了一个新的人类基因DBP-5。该cDNA克隆包含一个预测的开放阅读框，编码一个潜在的1,179个氨基酸的蛋白质。与DBP-5相对应的mRNA普遍分布，该基因在系统发育上是保守的。对几种细胞系的免疫荧光分析表明，该蛋白定位于细胞核。序列分析揭示了预测蛋白结构的不寻常特征，包括四个完全保守的重复序列。DBP-5的系统发育保守性、其表达的普遍性、其核定位以及其结合DNA序列的能力，提高了DBP-5可能在间期染色质的组织和/或转录调控中发挥作用的可能性。
In an effort to identify trans-acting factors regulating specific genes, we cloned a novel human gene, DBP-5. The cDNA clone contains a predicted open reading frame coding for a potential 1,179 amino acid protein. The mRNA corresponding to DBP-5 is ubiquitously distributed, and the gene is phylogenetically conserved. Immunofluorescence analyses with several cell lines indicate that the protein is localized to the nucleus. Sequence analysis revealed unusual features of the predicted protein structure, including four completely conserved repeats. The phylogenetic conservation of DBP-5, the ubiquity of its expression, its nuclear localization, and its ability to bind DNA sequences, raise the possibility that DBP-5 may play a role in the organization of interphase chromatin and/or in transcriptional regulation.