Formation of a Giant Toroid from Long Duplex DNA
Formation of a Giant Toroid from Long Duplex DNA
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DOI：
10.1021/la981159g
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发表时间：
1999-03
期刊：
影响因子：
3.9
通讯作者：
Y. Yoshikawa;K. Yoshikawa;T. Kanbe
中科院分区：
文献类型：
--
作者：
Y. Yoshikawa;K. Yoshikawa;T. Kanbe
众所周知，多胺如亚精胺具有将DNA分子凝聚成外径为80-100 nm的环形形态的作用。本文用荧光和电子显微镜研究了亚精胺诱导的长链T_4 DNA断裂。结果表明，在高盐缓冲溶液中，高浓度亚精胺作用下，单个T4 DNA可产生外径约为200 nm的巨大环状结构。
It is well-known that polyamines, such as spermidine, have the effect of condensing DNA molecules into a toroidal morphology with an outer diameter of 80-100 nm. We have investigated the collapse of long duplex T4 DNA induced by spermidine with use of fluorescence and electron microscopy. We found that giant toroids with an outer diameter of ∼200 nm are generated from single T4 DNAunder a high concentration of spermidine in the buffer solutions with rather high salt.