Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide
Directed differentiation and neurite extension of mouse embryonic stem cell on aligned poly(lactide) nanofibers functionalized with YIGSR peptide
复制标题
DOI：
10.1016/j.biomaterials.2013.08.028
复制
发表时间：
2013-12-01
期刊：
影响因子：
14
通讯作者：
Becker, Matthew L.
中科院分区：
文献类型：
--
作者：
Callahan, Laura A. Smith;Xie, Sibai;Becker, Matthew L.
将末端官能化的PLLA纳米纤维制造成随机或排列的纤维的垫，并使用肽Tyr-Ile-Gly-Ser-Arg（YIGSR）使用无金属的“点击化学”进行纺丝后官能化。发现与在随机纤维垫和未功能化基质上培养的小鼠胚胎干细胞（mESC）相比，用YIGSR对齐和功能化的纤维显著增加表达神经元特异性III类β-微管蛋白（TUJ 1）的小鼠胚胎干细胞（mESC）的分数、神经突延伸的水平和神经标记物的基因表达。可降解聚合物与生物活性肽的精确功能化创造了与预防相关的材料，这些材料利用了合成和天然系统的优势，同时减轻了各自的经典局限性。(C)2013爱思唯尔有限公司保留所有权利。
End-functional PLLA nanofibers were fabricated into mats of random or aligned fibers and functionalized post-spinning using metal-free "click chemistry" with the peptide Tyr-Ile-Gly-Ser-Arg (YIGSR). Fibers that were both aligned and functionalized with YIGSR were found to significantly increase the fraction of mouse embryonic stem cells (mESC) expressing neuron-specific class III beta-tubulin (TUJ1), the level of neurite extension and gene expression for neural markers compared to mESC cultured on random fiber mats and unfunctionalized matrices. Precise functionalization of degradable polymers with bioactive peptides created translationally-relevant materials that capitalize on the advantages of both synthetic and natural systems, while mitigating the classic limitations of each. (C) 2013 Elsevier Ltd. All rights reserved.