Quantitative studies of relationships between tumor cell ploidy and dose response to ionizing radiation in vivo.
Quantitative studies of relationships between tumor cell ploidy and dose response to ionizing radiation in vivo.
复制标题
肿瘤细胞倍性与体内电离辐射剂量反应之间关系的定量研究。
DOI：
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发表时间：
1963
期刊：
影响因子：
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通讯作者：
R. Berry
中科院分区：
文献类型：
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作者：
R. Berry
在两种密切相关的小鼠淋巴细胞白血病（一种二倍体和另一种四倍体）之间的体内移植被证明。在氧合和缺氧条件下测定剂量-反应曲线;在任一条件下，剂量-反应曲线的斜率相似，但四倍体肿瘤的外推数超过二倍体肿瘤约2倍。将二倍体来源的肿瘤细胞系在体内连续传代50代，每代接受1000拉德的X射线照射，每代移植10/ sup 6/肿瘤细胞，以最大限度地减少从少量存活细胞中选择的机会。在50次辐照传代期间，注意到该肿瘤系具有改变的辐射反应，在任何给定的辐射剂量下存活的肿瘤细胞比例更大，观察到每个细胞的核酸含量增加，并且在群体中出现四倍体和非整倍体细胞。这些研究结果的应用程序，以获得放射治疗师在治疗人类癌症的放射抗性报告进行了讨论。（auth）
ggradiation was demonstrated in vivo between two closely related murine lymphocytic leukemias, one diploid and the other tetraploid. Dose-response curves were determined under oxygenated and anoxic conditions; in either condition the slopes of the dose-response curves were similar, but the extrapolation number for the tetraploid tumor exceeded that for the diploid tumor by a factor of approximately 2. A tumor line of diploid origin was subjected to 1000 rads of x irradiation at each of fifty sequential passages in vivo with 10/ sup 6/ tumor cells transplanted at each passage to minimize the chance of selection from small numbers of surviving celis. During the fifty irradiated passages, this tumor line was noted to have altered radiation response with a greater proportion of tumor cells surviving any given radiation dose, increased nucleic acid content per cell was seen, and tetraploid and aneuploid cells appeared in the population. The application of these findings to the acquired radioresistance reported by radiotherapists in the treatment of human cancer is discussed. (auth)