Upper respiratory tract resistance to influenza infection is not prevented by the absence of either nasal-associated lymphoid tissue or cervical lymph nodes
Upper respiratory tract resistance to influenza infection is not prevented by the absence of either nasal-associated lymphoid tissue or cervical lymph nodes
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DOI：
10.4049/jimmunol.175.5.3186
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发表时间：
2005-09-01
影响因子：
4.4
通讯作者：
Harmsen, AG
中科院分区：
文献类型：
--
作者：
Wiley, JA;Tighe, MP;Harmsen, AG
小鼠鼻相关淋巴组织（NALT）和颈部淋巴结（CLN）参与上呼吸道（URT）内局部免疫反应的产生。然而，它们参与这些反应并不意味着对URT感染产生耐药性的必要性。我们通过手术切除NALT或CLN，以解决这些淋巴组织在URT流感感染后产生局部保护性免疫应答的必要性。在手术和随后的感染后，没有检测到任何组织重建的组织学证据。去除NALT不会引起血清或鼻粘膜相关流感特异性IG水平的变化。然而，PR 8特异性血清IgG和鼻粘膜相关伊加的增加后，检测去除CLN。流感特异性CD 4 T细胞向鼻粘膜的募集并没有因为去除NALT而改变。NALT或CLN的去除没有改变流感特异性CD 8 T细胞向URT的募集。然而，CLN手术后，在气管-支气管淋巴结中观察到流感特异性CD 8 T细胞水平增加。去除NALT或CLN不会改变鼻粘膜和肺的病毒清除率。这些研究表明，尽管NALT和CLN参与产生对流感感染的局部免疫力，但这两种组织对流感病毒的感染都不是必需的。在URT中发展保护性免疫和病毒清除。
The murine nasal-associated lymphoid tissue (NALT) and cervical lymph nodes (CLN) are involved in the generation of local immune responses within the upper respiratory tract (URT). However, their involvement in these responses does not imply the necessity for resistance to URT infections. We surgically removed NALT or CLN to address the necessity of these lymphatic tissues for the development of a local protective immune response after a URT influenza infection. No histological evidence of the re-establishment of either tissue was detected after surgery and the subsequent infection. Removal of NALT did not elicit changes in serum or nasal mucosa-associated influenza-specific Ig levels. However, increases in PR8-specific serum IgG and nasal mucosa-associated IgA were detected after removal of CLN. Recruitment of influenza-specific CD4 T cells into the nasal mucosa was not altered by removal of NALT. The removal of NALT or CLN did not alter the recruitment of influenza-specific CD8 T cells into the URT. However, increased levels of influenza-specific CD8 T cells were observed in the tracheal-bronchial lymph nodes after CLN surgery. The rate of viral clearance from nasal mucosa and lungs was not altered by removal of NALT or CLN. These studies demonstrate that despite the participation of NALT and CLN in the generation of local immunity to influenza infections, neither tissue is essential for the. development of protective immunity and viral clearance in URT.