Acute effect of ischemia on adrenomedullin immunoreactivity in the rat heart: an immunocytochemical study.
Acute effect of ischemia on adrenomedullin immunoreactivity in the rat heart: an immunocytochemical study.
复制标题
缺血对大鼠心脏肾上腺髓质素免疫反应性的急性影响：一项免疫细胞化学研究。
DOI：
10.3892/ijmm.14.1.71
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发表时间：
2004
影响因子：
5.4
通讯作者：
G. Nussdorfer
中科院分区：
文献类型：
--
作者：
A. Belloni;D. Guidolin;S. Ceretta;S. Bova;G. Nussdorfer
我们通过免疫细胞化学 (ICC) 研究了缺血对大鼠心脏肾上腺髓质素 (AM) 分布的急性影响，肾上腺髓质素是一种强效降血压肽，在心血管系统中表达，已知在心血管系统中发挥主要的调节和保护作用。从成年雄性 Sprague-Dawley 大鼠中收集心脏，用 Langendorff 技术进行灌注，并通过停止灌注 20 分钟获得“整体”缺血。冷冻心脏，并使用特异性抗大鼠 AM1-50 抗体和过氧化物酶偶联二抗进行 ICC。 ICC 在心肌细胞，尤其是冠状血管壁中证明了 AM 免疫反应性 (IR)。定量密度测定显示，急性缺血显着降低冠状动脉中的 AM-IR，从而表明它显着刺激 AM 释放。结论是急性缺血和随之而来的缺氧会激活大鼠心脏中AM的释放，AM通过其冠状扩张作用可以增强心脏血流量。
We investigated by immunocytochemistry (ICC) the acute effects of ischemia on the distribution in the rat heart of adrenomedullin (AM), a potent hypotensive peptide which is expressed in the cardiovascular system, where it is known to play a major regulatory and protective role. Hearts, collected from adult male Sprague-Dawley rats, were perfused with the Langendorff technique, and "global" ischemia was obtained by stopping perfusion for 20 min. Hearts were frozen, and ICC was performed using a specific anti-rat AM1-50 antibody and secondary peroxidase-conjugated antibodies. ICC demonstrated AM-immunoreactivity (IR) in cardiomyocytes and especially in the wall of coronary vessels. Quantitative densitometry showed that acute ischemia significantly decreased AM-IR in coronary arterioles, thereby suggesting that it markedly stimulates AM release. The conclusion is drawn that acute ischemia and ensuing hypoxia activate in the rat heart the release of AM, which by its coronarodilatory action may enhance heart blood flow.