MicroRNA profiling: from dark matter to white matter, or identifying new players in neurobiology.
MicroRNA profiling: from dark matter to white matter, or identifying new players in neurobiology.
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DOI：
10.1100/tsw.2007.201
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发表时间：
2007-11-02
影响因子：
--
通讯作者：
Krichevsky AM
中科院分区：
文献类型：
--
作者：
Krichevsky AM
最近发现的一类小调节RNA分子--微小RNA（miRNA）彻底改变了当代生物学。几十年来，由于其微小的尺寸，通常映射到基因组的非蛋白质编码区域，miRNA和miRNA介导的调控网络一直被研究人员错过，成为分子生物学的“暗物质”。在复杂生物体的中枢神经系统中破译miRNA通路和功能与理解miRNA表达模式紧密相关。为了促进这些新兴的研究，我在这里审查的基本原则，中，高通量技术可用于miRNA表达谱。
Contemporary biology has been revolutionized by a recently discovered class of small regulatory RNA molecules, microRNAs (miRNAs). Missed by researchers for decades due to their tiny size, usually mapping to non-protein-coding regions of genomes, miRNAs and miRNA-mediated regulatory networks have been the “dark matter” of molecular biology. Deciphering miRNA pathways and functions in the CNS of complex organisms is tightly linked to understanding miRNA expression patterns. To facilitate these emerging studies, I here review the basic principles of medium- and high-throughput technologies available for miRNA expression profiling.