The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance
The plant cuticle regulates apoplastic transport of salicylic acid during systemic acquired resistance
复制标题
DOI：
10.1126/sciadv.aaz0478
复制
发表时间：
2020-05-01
期刊：
影响因子：
13.6
通讯作者：
Kachroo, Pradeep
中科院分区：
文献类型：
--
作者：
Lim, Gah-Hyun;Liu, Huazhen;Kachroo, Pradeep
植物角质层通常被认为是环境的被动屏障。我们表明，角质层调节主动运输的防御激素水杨酸（SA）。SA是系统获得抗性（SAR）的重要调节因子，它优先通过质外体从病原菌侵染部位转运到未侵染部位。SA的质外体积累先于其在细胞质中的积累，其由SA的pH梯度和去质子化驱动。在角质层缺陷的突变体，增加蒸腾和降低水势优先路由SA角质层蜡，而不是质外体。这导致SA的长距离运输缺陷，这反过来又损害了SAR诱导剂哌啶酸的远端积累。高湿度减少蒸腾恢复系统SA运输，从而，SAR角质缺陷突变体。总之，我们的研究结果表明，SA的长距离流动性是必不可少的SAR和SA之间的共质体和角质层的分配是受蒸腾调节。
The plant cuticle is often considered a passive barrier from the environment. We show that the cuticle regulates active transport of the defense hormone salicylic acid (SA). SA, an important regulator of systemic acquired resistance (SAR), is preferentially transported from pathogen-infected to uninfected parts via the apoplast. Apoplastic accumulation of SA, which precedes its accumulation in the cytosol, is driven by the pH gradient and deprotonation of SA. In cuticle-defective mutants, increased transpiration and reduced water potential preferentially routes SA to cuticle wax rather than to the apoplast. This results in defective long-distance transport of SA, which in turn impairs distal accumulation of the SAR-inducer pipecolic acid. High humidity reduces transpiration to restore systemic SA transport and, thereby, SAR in cuticle-defective mutants. Together, our results demonstrate that long-distance mobility of SA is essential for SAR and that partitioning of SA between the symplast and cuticle is regulated by transpiration.