Interaction in vivo between the Two Matrix Attachment Regions Flanking a Single Chromatin Loop
Interaction in vivo between the Two Matrix Attachment Regions Flanking a Single Chromatin Loop
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DOI：
10.1016/j.jmb.2008.12.022
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发表时间：
2009-03-06
影响因子：
5.6
通讯作者：
Vassetzky, Yegor S.
中科院分区：
文献类型：
--
作者：
Elvazova, Elvira R.;Gavrilov, Aleksey;Vassetzky, Yegor S.
在间期核中，如中期染色体中，基因组被组织成拓扑闭合环域。在这里，我们已经在原代和培养的小鼠T淋巴细胞中定位了包含IFNG(干扰素-伽马)基因的环状结构域的末端。为了确定环的末端在核空间中是否彼此靠近，使用了检测蛋白质介导的DNA-DNA相互作用的染色体构象捕获(3C)技术。环的两端之间显示出强烈的相互作用，在存在对甲醛的情况下，环的两端足够近，可以在体内形成交联。染色质免疫沉淀结合3C技术表明，拓扑异构酶IIα和MeCP2参与了这种相互作用，而不是拓扑异构酶IIβ、异染色质相关蛋白HP1或CTCF参与了这种相互作用。就可导致染色体易位和缺失的非法重组机制而言，本研究结果具有重要意义。(C)2008爱思唯尔有限公司。保留所有权利。
In interphase nuclei as in metaphase chromosomes, the genome is organized into topologically closed loop domains. Here, we have mapped the ends of the loop domain that contains the Ifng (interferon-gamma) gene in primary and cultured murine T-lymphocytes. To determine whether the ends of the loop are located in close proximity to each other in the nuclear space, the 3C (chromosome conformation capture) technique, which detects protein-mediated DNA-DNA interactions, was utilized. A strong interaction was demonstrated between the two ends of the loop, which were close enough to become cross-linked in vivo in the presence of para formaldehyde. Chromatin immunoprecipitation combined with the 3C technique demonstrated that topoisomerase II alpha and MeCP2, but not topoisomerase II beta, heterochromatin-associated protein HP1 or CTCF, were involved in this interaction. The present findings have important implications, in terms of mechanisms of illegitimate recombination that can result in chromosomal translocations and deletions. (C) 2008 Elsevier Ltd. All rights reserved.