A novel beta-adrenergic like octopamine receptor modulates the audition of malaria mosquitoes and serves as insecticide target
A novel beta-adrenergic like octopamine receptor modulates the audition of malaria mosquitoes and serves as insecticide target
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DOI：
10.1101/2022.08.02.502538
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发表时间：
2022-08
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通讯作者：
M. Georgiades;CA Alampounti;J. Somers;M. Su;D. Ellis;J. Bagi;W. Ntabaliba;S. Moore;JT Albert;M. Andrés
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文献类型：
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作者：
M. Georgiades;CA Alampounti;J. Somers;M. Su;D. Ellis;J. Bagi;W. Ntabaliba;S. Moore;JT Albert;M. Andrés
听觉是疟蚊生命周期中必不可少的感觉。在黄昏时短暂形成的大群蚊子中，蚊子通过翅膀的拍打声来识别它们的交配伙伴。事实上，疟疾蚊子只在成群时对交配伙伴的飞行音做出反应。这种现象意味着蚊子听觉的复杂的环境和时间依赖性调节，其机制仍然很大程度上未知。利用转录组学，我们确定了调节蚊子听力的候选神经调节剂的复杂网络。其中，章鱼胺作为群体时期听觉表现的调节剂脱颖而出。为了探索章鱼胺在蚊子听力中的作用，我们深入分析了章鱼胺介导的对听觉功能的影响。我们发现章鱼胺在多个层面上影响蚊耳的特性：它调节鞭毛声音接收器的调谐和硬度，并控制雄性蚊子触角纤维的竖立。我们发现两种不同的受体正在驱动章鱼胺的听觉作用，其中包括一种新型的β章鱼胺受体。我们还证明了章鱼胺能听觉控制系统可以成为杀虫剂的目标。我们的研究结果表明，章鱼胺信号传导是疟疾蚊子听力和交配伙伴检测的关键组成部分，也是蚊子控制的潜在新目标。
Hearing is an essential sense in the life cycle of malaria mosquitoes. Within large swarms formed transiently at dusk, mosquitoes acoustically recognize their mating partners by their wingbeats. Indeed, malaria mosquitoes only respond to the flight tones of mating partners during swarm time. This phenomenon implies a sophisticated context- and time-dependent modulation of mosquito audition, the mechanisms of which are still largely unknown. Using transcriptomics, we identify a complex network of candidate neuromodulators regulating mosquito hearing. Among them, octopamine stands out as regulator of the auditory performance during swarm time. To explore octopamine’s roles in mosquito hearing, we carried out an in-depth analysis of octopamine-mediated effects on auditory function. We found that octopamine affects the properties of the mosquito ear on multiple levels: it modulates the tuning and stiffness of the flagellar sound receiver and it controls the erection of antennal fibrillae in males. We found that two different receptors are driving octopamine’s auditory roles, including a novel beta octopamine receptor. We also demonstrate that the octopaminergic auditory control system can be targeted by insecticides. Our findings identify octopamine signalling as a key component of hearing and mating partner detection in malaria mosquitoes, and as a potential novel target for mosquito control.