Genome-wide identification of stress-associated proteins (SAP) with A20/AN1 zinc finger domains associated with abiotic stresses responses in Brassica napus
Genome-wide identification of stress-associated proteins (SAP) with A20/AN1 zinc finger domains associated with abiotic stresses responses in Brassica napus
复制标题
具有与甘蓝型油菜非生物胁迫反应相关的 A20/AN1 锌指结构域的胁迫相关蛋白 (SAP) 的全基因组鉴定
DOI：
10.1016/j.envexpbot.2019.05.007
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发表时间：
2019-09-01
影响因子：
5.7
通讯作者：
Hua Wei
中科院分区：
文献类型：
--
作者：
He Xin;Xie Sai;Hua Wei
含有A20/AN1锌指结构域的胁迫相关蛋白(SAP)在植物非生物胁迫反应中发挥着重要作用。然而，甘蓝型油菜SAP基因家族(A(N)A(N)C(N))却鲜为人知。为了探索这些基因的分布和表达模式，对甘蓝型油菜SAP家族的基因进行了全基因组鉴定和系统分析。共鉴定出57个编码SAP的基因，并将其分为5个组。与在其他植物中发现的结果相反，进一步的基因结构和保守的蛋白质基序分析表明，Bna SAP(16/57)基因中有很大比例是A20型或AN1型SAP基因，其中大多数在白菜或甘蓝中没有发现同源基因。基于RNAseq数据，研究了Bna SAP启动子的顺式元件，以及Bna SAP基因在不同组织和不同胁迫处理下的表达水平。所有典型的A20-AN1型和AN1-AN1(-C2H2)型Bna SAP基因在所有组织中都有结构性表达，但II组中的4个成员除外。相反，几乎所有A20型和AN1型成员在所有获得转录组数据的受试油菜组织中都显示出很低的检测水平。此外，大多数典型的A20-AN1型和AN1-AN1型Bna SAP基因被发现是由多重胁迫和植物激素诱导的。在已鉴定的Bna SAP基因中，组3的4个成员(Bna A07g15770D、Bna C06g14100D、Bna C04g27800D和Bna C08g24010D)在叶片中受到不同程度的低温胁迫诱导，在根中受到盐胁迫和聚乙二醇盐胁迫的诱导。第IV组的EnaA06g02460D和BnaC06g04440D由热应激诱导，而第V组的BnaA06g31640D和BnaC07g25010D由热应激和水杨酸(SA)处理诱导。本研究对油菜SAP基因进行了全面的分析，可能有助于进一步研究A20/AN1类蛋白的功能以及油菜在各种非生物胁迫下的反应。
Stress-associated proteins (SAP) that contain the A20/AN1 zinc-finger domain play important roles in plants abiotic stress responses. However, little is known about the SAP gene family in Brassica napus (genome A(n)A(n)C(n)C(n)), was formed by recent allopolyploidy between ancestors of Brassica min (genome ArAr) and Brassica oleracea (genome CoCo). To explore the distribution and expression patterns of these genes, a genome-wide identification and a systematic analysis of genes in the SAP family in B. napus were performed. A total of 57 genes encoding SAP were identified, and subsequently classified into 5 groups. In contrast to results seen in other plant species, further analyses of gene structures and conserved protein motifs revealed that a large percentage of the BnaSAP (16/57) genes were A20-type or AN1-type SAP genes, most of which were not found to have homologous genes in B. rapa or B. oleracea. The cis-elements of the BnaSAP promoters, and BnaSAP gene expression levels in different tissues, and under different stress treatments, were also investigated based on RNAseq data. All the typical A20-AN1-type and AN1-AN1(-C2H2)-type BnaSAP genes were constitutively expressed among all tissues, except 4 members in Group II. In contrast, almost all A20 type and AN1 type members showed very low detection levels in all the tested rapeseed tissues from which the transcriptome data was derived. Additionally, most of the typical A20-AN1-type and AN1-AN1-type BnaSAP genes were found to be induced by multiple stresses and phytohormones. Among the identified BnaSAP genes, four members of Group III (BnaA07g15770D, BnaC06g14100D, BnaC04g27800D, and BnaC08g24010D) were induced following various low-temperature stresses in leaves, and were induced by NaCl and PEG stress in roots. EnaA06g02460D and BnaC06g04440D from Group IV were induced by heat stress, while BnaA06g31640D and BnaC07g25010D from Group V were induced by heat stress and salicylic acid (SA) treatment. This study provides a comprehensive analysis of SAP genes in rapeseed, and may aid in future efforts to identify the functions of A20/AN1-type proteins and the responses of rapeseed under a variety of abiotic stresses.