hsBAFF-Upregulated Intracellular Free Ca2+ Homeostasis Regulates ERK1/2 Activity and Cell Proliferation in B cells in Vitro
hsBAFF-Upregulated Intracellular Free Ca2+ Homeostasis Regulates ERK1/2 Activity and Cell Proliferation in B cells in Vitro
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DOI：
10.33549/physiolres.931522
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发表时间：
2009-01-01
影响因子：
2.1
通讯作者：
Chen, L.
中科院分区：
文献类型：
--
作者：
Liang, J. Q.;Zhang, W.;Chen, L.
我们研究了hsBAFF在体外小鼠脾B细胞中的活性。用荧光探针Fluo-3/AM检测hsBAFF对细胞内游离Ca ~（2+）浓度（[Ca ~（2+）]（i））的影响。我们发现，用0.5-5 μ g/ml hsBAFF处理B细胞，分别在12和24小时以剂量依赖的方式导致显著更高的[Ca 2 +]（i）水平（p
We studied hsBAFF activity in in vitro mouse splenic B cells. hsBAFF effects on intracellular free Ca2+ concentration ([Ca2+](i)) were assayed, using a laser scanning confocal microscope with fluorescent probe, Fluo-3/AM. We showed that treatment of B cells with 0.5-5 mu g/ml hsBAFF resulted in significantly higher [Ca2+](i) levels in a dose-dependent fashion at 12 and 24 h, respectively (p