Expression of the dietary isotope signal in the compound-specific δ13 values of pig bone lipids and amino acids
Expression of the dietary isotope signal in the compound-specific δ13 values of pig bone lipids and amino acids
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DOI：
10.1002/oa.658
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发表时间：
2003-01-01
影响因子：
1
通讯作者：
Evershed, RP
中科院分区：
文献类型：
--
作者：
Howland, MR;Corr, LT;Evershed, RP
猪饲养六同位素控制饮食，以检查用于合成散装骨化学成分（磷灰石，胶原蛋白和脂质）和个别化合物（骨脂肪酸，胆固醇和氨基酸胶原蛋白）的膳食常量营养素。磷灰石和大量骨脂的Δ C-13值反映了整个饮食的Δ C-13值，其中Δ C-13（磷灰石-整个饮食）=10.2 +/-1.3%和Δ C-13（骨脂-整个饮食）=-2.4 +/-0.7%。假设Delta C-13（胶原蛋白全饲料）值（0.5至6.1%）存在很大差异，以反映（i）必需氨基酸的直接掺入和（ii）直接掺入之间的平衡的相对重要性。从头合成非必需氨基酸。使用线性回归（n=6）来评估全饮食和散装骨组分的Δ C-13值与单个化合物之间的关系。整个饮食Δ C-13值显示出与骨胆固醇（R-2=0.85）和胶原中的谷氨酸（R-2=0.96）的强相关性。必需氨基酸亮氨酸（Delta（13）C（胶原蛋白leu diet leu）0.5 +/-1.2%）和苯丙氨酸（Delta C-13（胶原蛋白phe-diet phe）=-0.6 +/-0.6%）在饮食和骨胶原之间显示出很少的同位素分馏。
Pigs were raised on six isotopically controlled diets to examine the dietary macronutrients used in the synthesis of bulk bone isochemical components (apatite, collagen and lipids) and individual compounds (bone fatty acids, cholesterol and amino acids from collagen). delta C-13 values of apatite and bulk bone lipids reflected those of the whole diet, with Delta C-13(apatite-whole diet)=10.2 +/-1.3% and Delta C-13(bone lipids-whole diet)=-2.4 +/-0.7%. A wide variation observed in the Delta C-13(collagen-whole diet) values (0.5 to 6.1%) was hypothesized to reflect the relative importance of (i) the direct incorporation of essential amino acids, and (ii) the balance between direct incorporation and de novo synthesis of non-essential amino acids. Linear regression (n=6) was used to assess the relationship between the delta C-13 values of whole diet and bulk bone components and individual compounds. Whole diet delta C-13 values showed a strong correlation with those of bone cholesterol (R-2=0.85) and glutamate (R-2=0.96) in collagen. The essential amino acids leucine (Delta (13)C(collagen leu diet leu)0.5 +/-1.2%) and phenylalanine (Delta C-13(collagen phe-diet phe)=-0.6 +/-0.6%) showed little isotopic fractionation between diet and bone collagen.